|
| Status |
Public on Jul 06, 2006 |
| Title |
Schizosaccharomyces Pombe gcn5 mutant compared to wt, KCl treatment,replicate 1 (slide 5) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Schizosaccharomyces pombe gcn5 mutant Hu0799 treated with KCl
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
Schizosaccharomyces pombe gcn5 mutant Hu0799 treated with 1M KCl for 60min at 25C.
|
| Treatment protocol |
Schizosaccharomyces pombe gcn5 mutant Hu0799 treated with 1M KCl for 60min at 25C.
|
| Growth protocol |
Strain was grown on YEA medium at 25C to log phase then treated with 1M KCl for 60min.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| Label |
cy3
|
| Label protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| |
|
| Channel 2 |
| Source name |
Schizosaccharomyces pombe wild type strain fy368 treated with KCl for 60min
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
Schizosaccharomyces pombe wild type strain fy368 treated with 1M KCl for 60min.
|
| Treatment protocol |
Schizosaccharomyces pombe wild type strain fy368 treated with 1M KCl for 60min.
|
| Growth protocol |
Strain was grown on YEA medium at 25C to log phase then treated with 1M KCl for 60min.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| Label |
Cy5
|
| Label protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| |
|
| |
| Hybridization protocol |
See reference: Xue, Y. et al. A DNA microarray for fission yeast: minimal changes in global gene expression after temperature shift. Yeast 21, 25-39 (2004).
|
| Scan protocol |
Slides were scanned using Bio-rad Vers-Array ChipReader.
|
| Description |
Expression profile of Schizosaccharomyces pombe gcn5 mutant strain was compared to wild type strain, both treated with 1M KCl in rich medium at 25C.
|
| Data processing |
Scanned slides were quantified with Imagene 4.2 software. Quantified data were loaded to GeneSpring software and normalized by lowess normalization.
|
| |
|
| Submission date |
Jul 03, 2006 |
| Last update date |
Aug 24, 2009 |
| Contact name |
Anthony Wright |
| E-mail(s) |
yongtao.xue.franzen@ki.se
|
| Organization name |
Karolinska Institute
|
| Lab |
Anthony Wright
|
| Street address |
Blickagången 6
|
| City |
Huddinge |
| ZIP/Postal code |
141 57 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL3425 |
| Series (3) |
| GSE5218 |
Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe |
| GSE5227 |
Stress-specific role of the fission yeast Gcn5 acetylatransferase in programming a subset of stress-response genes |
| GSE16556 |
Role of Gcn5 in KCl stress adaptation in S. cerevisiae and S. pombe: transcriptional and genomic analyses |
|
