NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1186828 Query DataSets for GSM1186828
Status Public on Sep 01, 2013
Title OVCAR3-533533-R1_Selected_Drug_Rep1
Sample type genomic
 
Source name ovarian cancer cell line
Organism Homo sapiens
Characteristics cell type: Resistant
Treatment protocol Cell pellets were collected from subconfluent cultures, frozen and stored at -80ºC prior to nucleic acid extraction.
Growth protocol Cells were maintained at 37ºC and 5% CO2 in RPMI 1640 containing 10% (v/v) FCS, 50 U.mL-1 penicillin and 50ug.mL-1 streptomycin. 'Selected' cells were cultured in 4uM PHA-533533.
Extracted molecule genomic DNA
Extraction protocol Total genomic DNA was extracted using a DNeasy Kit (Qiagen, Valencia, CA)
Label C-Bio and A-DNP
Label protocol Infinium HD Assay was performed according to the manufacturer's instructions (Illumina)
 
Hybridization protocol Infinium HD Assay was performed using the BeadArray system according to the manufacturer's instructions (Illumina)
Scan protocol Infinium HD Assay was performed using the BeadArray system according to the manufacturer's instructions (Illumina)
Data processing Data was extracted using Illumina’s GenomeStudio v2010.3 with Genotyping module 1.8.4 software, with the default Illumina settings and Illumina HumanOmniExpress‐12v1_H manifest cluster file
 
Submission date Jul 16, 2013
Last update date Sep 01, 2013
Contact name Dariush Etemadmoghadam
E-mail(s) dariush.etemadmoghadam@petermac.org
Phone 61396561149
Organization name Peter MacCallum Cancer Centre
Street address St Andrew's Place
City East Melbourne
ZIP/Postal code 3002
Country Australia
 
Platform ID GPL13135
Series (2)
GSE48920 Copy number analysis of OVCAR-3 and CDK2 resistant sublines
GSE48921 Gene expression and copy number analysis of OVCAR-3 and CDK2 resistant sublines

Data table header descriptions
ID_REF
VALUE Normalised log R ratios used for copy number analysis provided in matrix file

Data table
ID_REF VALUE
rs1000000 -0.0332567
rs1000002 -0.0619671
rs10000023 -0.0461318
rs1000003 -0.0363029
rs10000030 0.568647
rs10000037 0.0686801
rs10000041 -0.141924
rs10000042 0.0121374
rs10000049 0.162884
rs1000007 -0.0294744
rs10000073 -0.0913779
rs10000081 0.0915786
rs10000092 0.0611722
rs10000105 0.210759
rs10000119 0.0834797
rs10000124 0.17166
rs10000154 0.0465215
rs1000016 -0.180149
rs10000160 -0.117744
rs10000169 0.288526

Total number of rows: 730525

Table truncated, full table size 14212 Kbytes.




Supplementary file Size Download File type/resource
GSM1186828_ILOE-6544_FinalReport2.txt.gz 27.1 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap