|
| Status |
Public on Sep 01, 2013 |
| Title |
OVCAR3-533533-R5_Selected_Drug_Rep1 |
| Sample type |
genomic |
| |
|
| Source name |
ovarian cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Resistant
|
| Treatment protocol |
Cell pellets were collected from subconfluent cultures, frozen and stored at -80ºC prior to nucleic acid extraction.
|
| Growth protocol |
Cells were maintained at 37ºC and 5% CO2 in RPMI 1640 containing 10% (v/v) FCS, 50 U.mL-1 penicillin and 50ug.mL-1 streptomycin. 'Selected' cells were cultured in 4uM PHA-533533.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total genomic DNA was extracted using a DNeasy Kit (Qiagen, Valencia, CA)
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
Infinium HD Assay was performed according to the manufacturer's instructions (Illumina)
|
| |
|
| Hybridization protocol |
Infinium HD Assay was performed using the BeadArray system according to the manufacturer's instructions (Illumina)
|
| Scan protocol |
Infinium HD Assay was performed using the BeadArray system according to the manufacturer's instructions (Illumina)
|
| Data processing |
Data was extracted using Illumina’s GenomeStudio v2010.3 with Genotyping module 1.8.4 software, with the default Illumina settings and Illumina HumanOmniExpress‐12v1_H manifest cluster file
|
| |
|
| Submission date |
Jul 16, 2013 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Dariush Etemadmoghadam |
| E-mail(s) |
dariush.etemadmoghadam@petermac.org
|
| Phone |
61396561149
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Street address |
St Andrew's Place
|
| City |
East Melbourne |
| ZIP/Postal code |
3002 |
| Country |
Australia |
| |
|
| Platform ID |
GPL13135 |
| Series (2) |
| GSE48920 |
Copy number analysis of OVCAR-3 and CDK2 resistant sublines |
| GSE48921 |
Gene expression and copy number analysis of OVCAR-3 and CDK2 resistant sublines |
|
