|
| Status |
Public on Jul 16, 2013 |
| Title |
Schisto-PH Mouse #2 |
| Sample type |
RNA |
| |
|
| Source name |
Schistosoma-PH mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
gender: female
disease state: experimental Schistosoma-induced pulmonary hypertension
tissue: whole lung
|
| Treatment protocol |
Control group: none. Schisto-PH group: 240 S. mansoni eggs IP/gram body weight, followed 2 weeks later by 240 S. mansoni eggs IV/gram body weight (tail vein injection), followed 1 week later by tissue harvest.
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy kit, Ambion WT Expression Kit #4411974
|
| Label |
Biotin
|
| Label protocol |
Affymetrix Genechip WT Terminal Label and Control Kit #901524
|
| |
|
| Hybridization protocol |
Affymetrix Hybridization/Wash/Stain Kit #900720
|
| Scan protocol |
Affymetrix GCS3000
|
| Description |
Schisto-PH 2
|
| Data processing |
CEL files pre-processed using GC-RMA algorithm in Affymetrix Expression Console.
|
| |
|
| Submission date |
Jul 16, 2013 |
| Last update date |
Jul 17, 2013 |
| Contact name |
Brian Graham |
| E-mail(s) |
brian.graham@ucdenver.edu
|
| Organization name |
University of Colorado Denver
|
| Department |
Medicine
|
| Street address |
12700 E 19th Ave, C-272
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE48936 |
Murine Schistosoma-Induced Pulmonary Hypertension: Microarray Data |
| GSE49116 |
Protective Role of IL6 in Vascular Remodeling in Schistosoma-Pulmonary Hypertension |
|
