|
| Status |
Public on Jul 19, 2013 |
| Title |
KHOS 3 |
| Sample type |
RNA |
| |
|
| Source name |
osteosarcoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: KHOS
metastatic: yes
|
| Treatment protocol |
Untreated
|
| Growth protocol |
DMEM/10%FCS/PSG, 5% CO2 for 24 h until 80% confluent
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with the Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol on an Illumina Human HT-12_V3_0_R1_11283641_A BeadChip
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were log2 transformed and normalised using quantile normalisation with lumi R package
|
| |
|
| Submission date |
Jul 18, 2013 |
| Last update date |
Jul 19, 2013 |
| Contact name |
Liliana Beatriz Endo-Munoz |
| E-mail(s) |
l.munoz@uq.edu.au
|
| Phone |
+61734436929
|
| Organization name |
University of Queensland
|
| Department |
Diamantina Institute
|
| Lab |
Saunders
|
| Street address |
TRI, 37 Kent St
|
| City |
Woolloongabba |
| State/province |
Queensland |
| ZIP/Postal code |
4102 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE49003 |
Gene expression analysis of metastatic and non-metastatic osteosarcoma cell lines |
|
