|
Status |
Public on Jul 19, 2013 |
Title |
KHOS 3 |
Sample type |
RNA |
|
|
Source name |
osteosarcoma cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: KHOS metastatic: yes
|
Treatment protocol |
Untreated
|
Growth protocol |
DMEM/10%FCS/PSG, 5% CO2 for 24 h until 80% confluent
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with the Agilent Bioanalyzer.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol on an Illumina Human HT-12_V3_0_R1_11283641_A BeadChip
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 3
|
Data processing |
The data were log2 transformed and normalised using quantile normalisation with lumi R package
|
|
|
Submission date |
Jul 18, 2013 |
Last update date |
Jul 19, 2013 |
Contact name |
Liliana Beatriz Endo-Munoz |
E-mail(s) |
l.munoz@uq.edu.au
|
Phone |
+61734436929
|
Organization name |
University of Queensland
|
Department |
Diamantina Institute
|
Lab |
Saunders
|
Street address |
TRI, 37 Kent St
|
City |
Woolloongabba |
State/province |
Queensland |
ZIP/Postal code |
4102 |
Country |
Australia |
|
|
Platform ID |
GPL6947 |
Series (1) |
GSE49003 |
Gene expression analysis of metastatic and non-metastatic osteosarcoma cell lines |
|