|
| Status |
Public on Jan 01, 2017 |
| Title |
CD3 lymphocytes from NM21692B |
| Sample type |
genomic |
| |
|
| Source name |
CD3 lymphocytes isolated from peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tissue/cell type: CD3 lymphocytes isolated from peripheral blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from whole bone marrow, whole peripheral blood or CD3 lymphocytes isolated from peripheral blood at diagnosis. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned according to the manufacturer's instructions.
|
| Description |
Hybridized to 6.0 Array
|
| Data processing |
CEL files, generated by AGCC Viewer. CNCHP and LOHCHP, generated by Genotyping Console (all provided by Affymetrix)
CNCHP (from 6.0 arrays) provide data for copy number and LOH analysis. LOCHP provides data for LOH analysis in case of 250K_Nsp arrays
|
| |
|
| Submission date |
Jul 18, 2013 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Mar Mallo |
| Organization name |
Josep Carreras Leukaemia Research Institute
|
| Street address |
Ctra de Can Ruti, s/n, camà de les escoles. Edifici IMPPC
|
| City |
Badalona |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE49004 |
Parallel SNP and CGH arrays of cytogenetic normal MDS patients and detection of alterations present in CD3+ T cells and bone marrow cells |
|
