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Sample GSM1192738 Query DataSets for GSM1192738
Status Public on Apr 25, 2014
Title Plants overexpressing WRKY6, arsenate 30μM, 1.5 hours. Rep2
Sample type RNA
 
Source name Plants overexpressing WRKY6, arsenate 30μM, 1.5 hours
Organism Arabidopsis thaliana
Characteristics ecotype: Col-0
genotype: OXW6
supplement: Arsenate (30μM)
time: 1.5 hours
Treatment protocol Plants were transferred to liquid medium supplemented with 30 μM As(V), 30 μM Pi, 1mM Pi (or not supplemented) for indicated times.
Growth protocol Plants were grown on Johnson medium with 1 mM Pi (7 days), transferred to -Pi medium (2 days).
Extracted molecule total RNA
Extraction protocol RNA was isolated from three independent biological samples for each condition using the RNeasy Plant Mini Kit (Qiagen). Each RNA preparation was tested for degradation using the Agilent 2100 Bioanalyzer (Agilent technologies, Palo Alto, CA).
Label Cy3
Label protocol cDNA was Cy3-labeled with the Nimblegen One-Color DNA Labeling Kit (Roche).
 
Hybridization protocol Each microarray was hybridized with the Nimblegen Hybridization Kit, washed using the Nimblegen Wash Buffer Kit (both from Roche) and scanned at 2 μm double-pass at 16 bit of resolution.
Scan protocol Scanned at 2 μm double-pass at 16 bit of resolution with the official Nimblegen scanner.
Description Plants overexpressing WRKY6 transcription factor supplemented with arsenate 30μM (1.5 hours). Replicate 2
Data processing Data were analyzed through Robust Multi-array Analysis (RMA) with Nimblegen Scan software (Roche).
 
Submission date Jul 19, 2013
Last update date Apr 25, 2014
Contact name Juan Carlos Oliveros
Organization name CNB, CSIC
Street address Darwin 3
City Cantoblanco
State/province Madrid
ZIP/Postal code 28049
Country Spain
 
Platform ID GPL13970
Series (1)
GSE49037 WRKY6 Transcription Factor Restricts Arsenate Uptake and Transposon Activation in Arabidopsis

Data table header descriptions
ID_REF
VALUE RMA-normalized log signal

Data table
ID_REF VALUE
AT1G01010_1 14.3701
AT1G01020_1 10.2687
AT1G01020_2 9.1226
AT1G01030_1 11.6856
AT1G01040_1 13.0284
AT1G01046_1 8.588
AT1G01050_1 15.478
AT1G01060_1 14.2625
AT1G01060_3 7.7893
AT1G01070_1 13.3408
AT1G01070_2 8.0055
AT1G01073_1 8.6577
AT1G01080_1 14.7004
AT1G01080_2 14.3521
AT1G01090_1 15.7965
AT1G01100_1 15.6342
AT1G01100_2 15.7357
AT1G01100_3 15.6629
AT1G01110_1 12.0792
AT1G01110_2 12.5112

Total number of rows: 37118

Table truncated, full table size 710 Kbytes.




Supplementary file Size Download File type/resource
GSM1192738_511839A11_OXW6-As-2_532.pair.gz 2.4 Mb (ftp)(http) PAIR
Processed data included within Sample table

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