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Sample GSM1204482 Query DataSets for GSM1204482
Status Public on Apr 21, 2015
Title WT sucrose x WT no stress- rep2
Sample type RNA
 
Channel 1
Source name WT- sucrose
Organism Caulobacter vibrioides NA1000
Characteristics strain: NA1000 cells
growth condition: under sucrose stress
Treatment protocol Cultures were kept under no stress condition or treated with either 110 mM sucrose or 60 mM NaCl
Growth protocol Overnight C. crescentus cultures were diluted to an optical density at 600 nm (OD600) of 0.1 in minimal salt medium supplemented with glucose and grown until midlog phase (OD600 ~ 0.5)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol Standard Cy3-dCTP and Cy5-dCTP incorporations standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation
 
Channel 2
Source name WT- no stress
Organism Caulobacter vibrioides NA1000
Characteristics strain: NA1000 cells
growth condition: under no stress condition
Treatment protocol Cultures were kept under no stress condition or treated with either 110 mM sucrose or 60 mM NaCl
Growth protocol Overnight C. crescentus cultures were diluted to an optical density at 600 nm (OD600) of 0.1 in minimal salt medium supplemented with glucose and grown until midlog phase (OD600 ~ 0.5)
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol Standard Cy3-dCTP and Cy5-dCTP incorporations standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation
 
 
Hybridization protocol Agilent protocol Agilent protocol Agilent protocol Agilent protocol
Scan protocol Agilent protocol Agilent protocol
Data processing Data were extracted and normalized using Agilent Feature Extraction Software 9.0. Agilent output files were then opened using Excell and the log10 values extracted with no additional processing or filtering
 
Submission date Aug 08, 2013
Last update date Apr 23, 2015
Contact name Christian Kohler
E-mail(s) kohlerc@uni-greifswald.de
Organization name Friedrich Loeffler Institute for Medical Microbiology
Department Medical Microbiology University Medicine
Street address Martin Luther Strasse 6
City Greifswald
ZIP/Postal code 17489
Country Germany
 
Platform ID GPL14163
Series (1)
GSE49654 A comprehensive genomic, transcriptomic and proteomic analysis of a hyperosmotic stress sensitive α-proteobacterium.

Data table header descriptions
ID_REF
VALUE Lowess normalized log10 ratio Cy3/Cy5 (test/ reference)

Data table
ID_REF VALUE
1 0.00
2 0.00
3 0.00
4 0.00
5 0.00
6 0.00
7 0.00
8 0.00
9 0.00
10 -0.10
11 0.00
12 -0.21
13 0.06
14 0.53
15 0.09
16 0.15
17 0.18
18 0.27
19 -0.08
20 0.02

Total number of rows: 45206

Table truncated, full table size 497 Kbytes.




Supplementary file Size Download File type/resource
GSM1204482_sucrose_stimulon-rep2.txt.gz 12.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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