|
| Status |
Public on Apr 21, 2015 |
| Title |
WT sucrose x WT no stress- rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT- sucrose
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
strain: NA1000 cells
growth condition: under sucrose stress
|
| Treatment protocol |
Cultures were kept under no stress condition or treated with either 110 mM sucrose or 60 mM NaCl
|
| Growth protocol |
Overnight C. crescentus cultures were diluted to an optical density at 600 nm (OD600) of 0.1 in minimal salt medium supplemented with glucose and grown until midlog phase (OD600 ~ 0.5)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Standard Cy3-dCTP and Cy5-dCTP incorporations standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation
|
| |
|
| Channel 2 |
| Source name |
WT- no stress
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
strain: NA1000 cells
growth condition: under no stress condition
|
| Treatment protocol |
Cultures were kept under no stress condition or treated with either 110 mM sucrose or 60 mM NaCl
|
| Growth protocol |
Overnight C. crescentus cultures were diluted to an optical density at 600 nm (OD600) of 0.1 in minimal salt medium supplemented with glucose and grown until midlog phase (OD600 ~ 0.5)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Standard Cy3-dCTP and Cy5-dCTP incorporations standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation standard Cy3-dCTP incorporation
|
| |
|
| |
| Hybridization protocol |
Agilent protocol Agilent protocol Agilent protocol Agilent protocol
|
| Scan protocol |
Agilent protocol Agilent protocol
|
| Data processing |
Data were extracted and normalized using Agilent Feature Extraction Software 9.0. Agilent output files were then opened using Excell and the log10 values extracted with no additional processing or filtering
|
| |
|
| Submission date |
Aug 08, 2013 |
| Last update date |
Apr 23, 2015 |
| Contact name |
Christian Kohler |
| E-mail(s) |
kohlerc@uni-greifswald.de
|
| Organization name |
Friedrich Loeffler Institute for Medical Microbiology
|
| Department |
Medical Microbiology University Medicine
|
| Street address |
Martin Luther Strasse 6
|
| City |
Greifswald |
| ZIP/Postal code |
17489 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14163 |
| Series (1) |
| GSE49654 |
A comprehensive genomic, transcriptomic and proteomic analysis of a hyperosmotic stress sensitive α-proteobacterium. |
|
