Additional Information: All 18 samples will be available 12/16/05. RNA was isolated from LCM samples using Arcturus\' PicoPure Isolation kit. Samples went through two amplifications using Arcturus\' Ribo Amp OA kit with an Affymetrix IVT label. Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ug Ratio: 1.81 RNA Integrity Number: 7.2 Technical Replicate: norvegicus Strain or Line: Sprague-Dawley rat NCBI Taxonomy ID: 10116 Developmental State: adult Sacrifice Method: 60 mg/kg sodium pentbarbital IP Sex: male Age: 60 Units of Age: days
Biomaterial provider
Elizabeth S. Roberts, CIIT Centers for Health Research, 6 Davis Drive, Research Triangle Park, NC, 27709, Elizabeth, eroberts@ciit.org, 558-1347, 558-1300
Treatment protocol
in vivo Treatment: H2S , Treatment Duration: 3, Units of Duration: hr, Treatment Time: 1, Units of Treatment Time: exposure, Route of Exposure: inhalation, Dose or Concentration: 200, Units of Dose or Concentration: ppm
Extracted molecule
total RNA
Extraction protocol
Extraction Method: PicoPure Nucleic acid spin column Additional Purification Method: Ribo Amp OA and Affymetrix IVT label
Label
biotin
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
ER_3_Rat230_2_IMF RT 0023: frozen sample for laser capture microdissection and RNA microarray analysis.
