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Sample GSM120724 Query DataSets for GSM120724
Status Public on Jul 01, 2008
Title epithelium_5: 3h; H2S
Sample type RNA
 
Source name 3h; H2S
Organism Rattus rattus
Characteristics Additional Information: All 18 samples will be available 12/16/05. RNA was isolated from LCM samples using Arcturus\' PicoPure Isolation kit. Samples went through two amplifications using Arcturus\' Ribo Amp OA kit with an Affymetrix IVT label.
Array Type: Rat230_2
Amount to Core: 15
Units of Amount: ug
Ratio: 1.83
Technical Replicate: norvegicus
Strain or Line: Sprague-Dawley rat
NCBI Taxonomy ID: 10116
Developmental State: adult
Sacrifice Method: 60 mg/kg sodium pentbarbital IP
Sex: male
Age: 60
Units of Age: days
Biomaterial provider Elizabeth S. Roberts, CIIT Centers for Health Research, 6 Davis Drive, Research Triangle Park, NC, 27709, Elizabeth, eroberts@ciit.org, 558-1347, 558-1300
Treatment protocol in vivo Treatment: H2S , Treatment Duration: 3, Units of Duration: hr, Treatment Time: 1, Units of Treatment Time: exposure, Route of Exposure: inhalation, Dose or Concentration: 200, Units of Dose or Concentration: ppm
Extracted molecule total RNA
Extraction protocol Extraction Method: PicoPure Nucleic acid spin column
Additional Purification Method: Ribo Amp OA and Affymetrix IVT label
Label biotin
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description ER_5_Rat230_2_IMF RT 0023: frozen sample for laser capture microdissection and RNA microarray analysis.
Data processing Affymetrix GCOS 1.0
 
Submission date Jul 17, 2006
Last update date Dec 30, 2007
Contact name Elizabeth Sherman Roberts
E-mail(s) elizabeth.roberts@novartis.com
Phone (336) 387-1487
Fax (336) 387-1459
Organization name Novartis Animal Health US
Street address 3200 Northline Ave, Suite 300
City Greensboro
State/province NC
ZIP/Postal code 27408
Country USA
 
Platform ID GPL1355
Series (1)
GSE5349 Gene Expression Changes in Laser Capture Microdissected Nasal Respiratory Epithelium of Rats Exposed to Hydrogen Sulfide

Data table header descriptions
ID_REF Affymetrix probe name
VALUE GCOS-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 1380.48 P 0.000972
AFFX-BioB-M_at 2613.04 P 4.4e-05
AFFX-BioB-3_at 1649.93 P 4.4e-05
AFFX-BioC-5_at 3572.24 P 4.4e-05
AFFX-BioC-3_at 3451.71 P 4.4e-05
AFFX-BioDn-5_at 8348.06 P 4.4e-05
AFFX-BioDn-3_at 13146.7 P 0.00011
AFFX-CreX-5_at 39595.2 P 5.2e-05
AFFX-CreX-3_at 40987.2 P 4.4e-05
AFFX-r2-Ec-bioB-5_at 1802.91 P 0.000244
AFFX-r2-Ec-bioB-M_at 2400.58 P 0.000244
AFFX-r2-Ec-bioB-3_at 2551.3 P 0.000244
AFFX-r2-Ec-bioC-5_at 5414.46 P 0.000244
AFFX-r2-Ec-bioC-3_at 6660.75 P 0.000244
AFFX-r2-Ec-bioD-5_at 23682 P 0.000244
AFFX-r2-Ec-bioD-3_at 26463.6 P 0.000244
AFFX-r2-P1-cre-5_at 53200.3 P 0.000244
AFFX-r2-P1-cre-3_at 67984.5 P 0.000244
AFFX-r2-Bs-phe-3_at 48.0491 P 0.008057
AFFX_Rat_GAPDH_5_at 220.359 P 0.000509

Total number of rows: 31099

Table truncated, full table size 905 Kbytes.




Supplementary data files not provided

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