|
| Status |
Public on Aug 15, 2013 |
| Title |
crypt |
| Sample type |
RNA |
| |
|
| Source name |
crypt
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
AGPC metnod for preparation of RNA extraction
|
| Label |
Cy3
|
| Label protocol |
40 ng total RNA from sample o- organoid and 20 ng total RNA from all other samples was used as template to produce Cy3- labeled cRNA. The RNA samples were amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Cy3 labeled cRNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Mouse Genome Oligo Microarrays (4x44K V2) using Agilent’s recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
|
| Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
|
| Description |
wild tyle
|
| Data processing |
The Agilent Feature Extraction Software (FES 10.7.3.1 ) was used to read out and process the microarray image files.
|
| |
|
| Submission date |
Aug 14, 2013 |
| Last update date |
Aug 15, 2013 |
| Contact name |
Toshio Takahashi |
| E-mail(s) |
takahashi@sunbor.or.jp
|
| Organization name |
Suntory Foundation for Life Sciences
|
| Street address |
1-1-1 Wakayamadai
|
| City |
Osaka |
| ZIP/Postal code |
618-8503 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10333 |
| Series (1) |
| GSE49864 |
Non-neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5-positive stem cells in mice |
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