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Sample GSM1208311 Query DataSets for GSM1208311
Status Public on Aug 15, 2013
Title crypt
Sample type RNA
Source name crypt
Organism Mus musculus
Characteristics strain: C57/BL6
Extracted molecule total RNA
Extraction protocol AGPC metnod for preparation of RNA extraction
Label Cy3
Label protocol 40 ng total RNA from sample o- organoid and 20 ng total RNA from all other samples was used as template to produce Cy3- labeled cRNA. The RNA samples were amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) following the manufacturer’s protocol.
Hybridization protocol Cy3 labeled cRNAs were hybridized overnight (17 hours, 65°C) to an Agilent Whole Mouse Genome Oligo Microarrays (4x44K V2) using Agilent’s recommended hybridization chamber and oven.Finally, microarrays were washed once with wash buffer 1 for 1 min at RT followed by a second wash with wash buffer 2 for 1 min at 37°C and a final wash step with acetonitrile for 30 sec at RT.
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA).
Description wild tyle
Data processing The Agilent Feature Extraction Software (FES ) was used to read out and process the microarray image files.
Submission date Aug 14, 2013
Last update date Aug 15, 2013
Contact name Toshio Takahashi
Organization name Suntory Foundation for Life Sciences
Street address 1-1-1 Wakayamadai
City Osaka
ZIP/Postal code 618-8503
Country Japan
Platform ID GPL10333
Series (1)
GSE49864 Non-neuronal acetylcholine as an endogenous regulator of proliferation and differentiation of Lgr5-positive stem cells in mice

Data table header descriptions
VALUE median normalized signal intensities

Data table
12 0.195452
13 0.19574
14 0.195966
15 0.196165
16 47.464148
17 65.704775
18 13.856265
19 0.196626
20 0.196713
21 2.8919
22 3.076743
23 1119.076687
24 83.786548
25 7.576354
26 0.197118
27 91.776247
28 754.057743
30 46.849749
31 132.218246
32 0.198046

Total number of rows: 43020

Table truncated, full table size 633 Kbytes.

Supplementary file Size Download File type/resource
GSM1208311_252665511299_S01_GE1_107_Sep09_1_4.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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