|
| Status |
Public on Aug 28, 2013 |
| Title |
Fetal mHeat-WT_E16.5_B6 |
| Sample type |
RNA |
| |
|
| Source name |
fetal mouse heart
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: wild type
age: embryonic day 16.5
tissue: Cardiac tissue
|
| Growth protocol |
CMPs were cultured on Collagen I coated dish in DMEM/F12 supplemented with 10% FBS, 40 ng/ml bFGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each samples using the RNeasy Plus Mini Kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
250 ng of total RNA was labelled with Cy3 using the Low RNA Fluorecent Linear Amplification Kit PLUS, One-Color (Agilent Technologies) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization and washing steps were using Gene Expression Wash Pack (Agilent Technology) according to the manufacturer's instructions.
|
| Scan protocol |
Fluorescence signals of the hybridized Agilent Microarrays were detected using DNA MicroArray Scanner (Agilent Technologies).
|
| Description |
6-Fetal mHeat-WT_E16.5_B6.txt
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction Software version 9.5.3 and further analyzed using GeneSpring GX software version 11.0.2. Data were normalized to the 75 th percentile (median).
|
| |
|
| Submission date |
Aug 27, 2013 |
| Last update date |
Aug 28, 2013 |
| Contact name |
Satoshi Gojo |
| E-mail(s) |
gojos@koto.kpu-m.ac.jp
|
| Phone |
+81-75-251-5511
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Street address |
465 Kajii-cho, Kamigyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE50221 |
Cardiac mesenchymal progenitors from postmortem cardiac tissue retained cellular characterization |
|
