|
| Status |
Public on Feb 01, 2014 |
| Title |
Postnatal 12 weeks mutant heart biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
postnatal 12 weeks pitx2 cko heart
|
| Organism |
Mus musculus |
| Characteristics |
age: 12 wk
tissue: whole heart
genotype: MCKcre Pitx2 Flox/Flox
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using Trizol (GIBCOyBRL). RNA pellets were resuspended at 1 ug RNA/ul diethyl pyrocarbonate-treated water.
|
| Label |
biontin
|
| Label protocol |
8 ug of total RNA was used in a reverse transcription reaction (SuperScript II; Life Technologies, Rockville, MD) to generate first-strand cDNA. After second-strand synthesis, double-strand cDNA was used in an in vitro transcription (IVT) reaction to generate biotinylated cRNA.
|
| |
|
| Hybridization protocol |
After purification and fragmentation, 15ug of cRNA was used in a 300 ul hybridization mixture containing spiked IVT controls. Approximately 200 ul of mixture was hybridized on chips for 16 h at 45°C. Standard posthybridization wash and double-stain protocols used an Affymetrix GeneChip Fluidics Station 400.
|
| Scan protocol |
Arrays were scanned by GeneChip Scanner 3000
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 28, 2013 |
| Last update date |
Feb 01, 2014 |
| Contact name |
Min Zhang |
| E-mail(s) |
minzhang5099@gmail.com
|
| Organization name |
Baylor College of Medicine
|
| Department |
Department of Molecular Physiology
|
| Lab |
James F Martin
|
| Street address |
One Baylor Plaza Room 504E
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE50399 |
Gene expression data of postnatal Pitx2 deficient heart |
| GSE50401 |
Pitx2 in the postnatal heart |
|
