|
Status |
Public on Sep 01, 2014 |
Title |
Colorectal Normal Tissue-030613-01NB-Affymetrix Human Exon 1.0 ST |
Sample type |
RNA |
|
|
Source name |
Colorectal Normal Tissue
|
Organism |
Homo sapiens |
Characteristics |
cell type: Adjacent Normal gender: Female age: 61 tissue: Colon & Rectum tnm: Not Available Stage: IV
|
Treatment protocol |
not applicable
|
Growth protocol |
NONE (Tissue samples were used)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from <30mg of biopsy and surgery derived tissues using Macherey Nagel (Germany) Trio Kit. Both DNA and RNA were extracted in the same protocol accoding to manufacturers protocol.
|
Label |
Biotin
|
Label protocol |
Gene Chip WT Terminal Labelling and Controls Kit (cat # 951024) was procured from Affymetrix and used according the manufacturers protocol.
|
|
|
Hybridization protocol |
GeneChip® Hybridization Wash and Stain Kit (cat # 900720) was procured from Affymetrix and used according the manufacturers protocol.
|
Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
|
Description |
RMA expression value derived from AltAnalyze; core-exon analysis
|
Data processing |
Data Processing using AltAnalyze Software. The default recommended settings by Altanalyze was used to analyze the data
|
|
|
Submission date |
Aug 28, 2013 |
Last update date |
Sep 01, 2014 |
Contact name |
Sathish Periyasamy |
E-mail(s) |
sathish.periyasamy@gmail.com
|
Phone |
0966503921073
|
Organization name |
NGHA
|
Department |
Bioinformatics
|
Street address |
P.O. Box 22490
|
City |
Riyadh |
State/province |
Select a State or Province |
ZIP/Postal code |
11426 |
Country |
Saudi Arabia |
|
|
Platform ID |
GPL5188 |
Series (1) |
GSE50421 |
Exon Array Data for Colerectal Tumor and Normal Samples |
|