|
| Status |
Public on Sep 01, 2014 |
| Title |
Colorectal Normal Tissue-032612-01NB-Affymetrix Human Exon 1.0 ST |
| Sample type |
RNA |
| |
|
| Source name |
Colorectal Normal Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Adjacent Normal
gender: Female
age: 56
tissue: Colon & Rectum
tnm: T3N0M0
Stage: II
|
| Treatment protocol |
not applicable
|
| Growth protocol |
NONE (Tissue samples were used)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from <30mg of biopsy and surgery derived tissues using Macherey Nagel (Germany) Trio Kit. Both DNA and RNA were extracted in the same protocol accoding to manufacturers protocol.
|
| Label |
Biotin
|
| Label protocol |
Gene Chip WT Terminal Labelling and Controls Kit (cat # 951024) was procured from Affymetrix and used according the manufacturers protocol.
|
| |
|
| Hybridization protocol |
GeneChip® Hybridization Wash and Stain Kit (cat # 900720) was procured from Affymetrix and used according the manufacturers protocol.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000 7G.
|
| Description |
RMA expression value derived from AltAnalyze; core-exon analysis
|
| Data processing |
Data Processing using AltAnalyze Software. The default recommended settings by Altanalyze was used to analyze the data
|
| |
|
| Submission date |
Aug 28, 2013 |
| Last update date |
Sep 01, 2014 |
| Contact name |
Sathish Periyasamy |
| E-mail(s) |
sathish.periyasamy@gmail.com
|
| Phone |
0966503921073
|
| Organization name |
NGHA
|
| Department |
Bioinformatics
|
| Street address |
P.O. Box 22490
|
| City |
Riyadh |
| State/province |
Select a State or Province |
| ZIP/Postal code |
11426 |
| Country |
Saudi Arabia |
| |
|
| Platform ID |
GPL5188 |
| Series (1) |
| GSE50421 |
Exon Array Data for Colerectal Tumor and Normal Samples |
|
