|
| Status |
Public on Sep 24, 2013 |
| Title |
HSP70K71E HEK293 sample3 |
| Sample type |
RNA |
| |
|
| Source name |
HEK293 cell line, expressing HSP70K71E
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293
genotype/variation: expressing HSP70K71E
|
| Growth protocol |
HEK293 cells and the cells expressing HSP70K71E were proliferated with RPMI1640, 10% FBS, 25 mM HEPES, 1X PS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested (RNAeasy kit(Qiagen)/ standard protocol as manufacture recommended) and measured the concentration for mRNA analysis
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Ambion MessageAmp II Biotin-enhanced protocol from 1 ug total RNA (Message Amp II Biotin-Enhanced Technical Manual, 2006, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133 plus 2 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 Laser Scanner.
|
| Description |
H3
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Sep 03, 2013 |
| Last update date |
Sep 24, 2013 |
| Contact name |
Jung S Byun |
| E-mail(s) |
byunjs@mail.nih.gov
|
| Phone |
3014352890
|
| Organization name |
NCI/NIH
|
| Lab |
Genetics Branch
|
| Street address |
37 convent drive, Bldg 37, Rm 1042
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE50547 |
Global mRNA gene expression analysis profiling control HEK293 cell and cells expressing HSP70K71E |
| GSE51118 |
p300 is a preferred client protein for Nuclear Chaperones |
|
