|
Status |
Public on Jul 31, 2006 |
Title |
15dpi rep A Control (Cy5) vs. Mycorrhiza (Cy3) (12770659) |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Pinus sylvestris root tissues rep A Control (Cy5)
|
Organism |
Pinus sylvestris |
Characteristics |
Pinus sylvestris unchallenged (control)
|
Treatment protocol |
Pinus sylvestris seedlings (14 days post-germination) were mock-inoculated with sterile distilled water and harvested at 15dpi. Total RNA was extracted and used for cDNA synthesis with SMART PCR cDNA synthesis kit (Clontech, USA).
|
Extracted molecule |
total RNA |
Label |
Cy5
|
Label protocol |
The cDNA generated from the roots was labelled using a Klenow method following the protocol from North Carolina State University (van Zyl, U.S. Provisional Patent Application Nos. 60/372,872 and 60/390,142).
|
|
|
Channel 2 |
Source name |
rep A Mycorrhiza (Cy3)
|
Organism |
Pinus sylvestris |
Characteristics |
Pinus sylvestris challenged for 15 days with: Mycorrhiza (Laccaria bicolor)
|
Treatment protocol |
Pinus sylvestris seedlings (14 days post-germination) were inoculated with homogenized mycelium of Laccaria bicolor and harvested at 15dpi. Total RNA was extracted and used for cDNA synthesis with SMART PCR cDNA synthesis kit (Clontech, USA).
|
Extracted molecule |
total RNA |
Label |
Cy3
|
Label protocol |
The cDNA generated from the roots was labelled using a Klenow method following the protocol from North Carolina State University (van Zyl, U.S. Provisional Patent Application Nos. 60/372,872 and 60/390,142).
|
|
|
|
Hybridization protocol |
Hybridization and stringency washes were done according to the protocol from North Carolina State University (van Zyl, U.S. Provisional Patent Application Nos. 60/372,872 and 60/390,142).
|
Description |
rep A Control (Cy5) vs. Mycorrhiza (Cy3) (12770659)
|
Data processing |
Raw intensity generated with QuantArray and statistically analysed using 2-interconnected mixed linear model approach.
|
|
|
Submission date |
Jul 28, 2006 |
Last update date |
Jul 31, 2006 |
Contact name |
Chris Smith |
Organization name |
NCSU
|
Department |
Bioinformatics Research Center
|
Lab |
1500 Partners II
|
Street address |
840 Main Campus Dr
|
City |
Raleigh |
State/province |
NC |
ZIP/Postal code |
27606 |
Country |
USA |
|
|
Platform ID |
GPL4039 |
Series (2) |
GSE5407 |
Comparative transcript profiling of interaction events in Pinus sylvestris root tissues following challenge (15dpi). |
GSE5410 |
Comparative transcriptome analysis of early interaction events in Scots pine root tissues following challenge |
|