|
| Status |
Public on Jul 31, 2006 |
| Title |
15dpi rep B Control (Cy5) vs. Pathogen (Cy3) (12780954) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Pinus sylvestris root tissues rep B Control (Cy5)
|
| Organism |
Pinus sylvestris |
| Characteristics |
Pinus sylvestris unchallenged (control)
|
| Treatment protocol |
Pinus sylvestris seedlings (14 days post-germination) were mock-inoculated with sterile distilled water and harvested at 15dpi. Total RNA was extracted and used for cDNA synthesis with SMART PCR cDNA synthesis kit (Clontech, USA).
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| Label protocol |
The cDNA generated from the roots was labelled using a Klenow method following the protocol from North Carolina State University (van Zyl, U.S. Provisional Patent Application Nos. 60/372,872 and 60/390,142).
|
| |
|
| Channel 2 |
| Source name |
rep B Pathogen (Cy3)
|
| Organism |
Pinus sylvestris |
| Characteristics |
Pinus sylvestris challenged for 15 days with: Pathogen (Heterobasidion annosum)
|
| Treatment protocol |
Pinus sylvestris seedlings (14 days post-germination) were inoculated with homogenized mycelium of Heterobasidion annosum and harvested at 15dpi. Total RNA was extracted and used for cDNA synthesis with SMART PCR cDNA synthesis kit (Clontech, USA).
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| Label protocol |
The cDNA generated from the roots was labelled using a Klenow method following the protocol from North Carolina State University (van Zyl, U.S. Provisional Patent Application Nos. 60/372,872 and 60/390,142).
|
| |
|
| |
| Hybridization protocol |
Hybridization and stringency washes were done according to the protocol from North Carolina State University (van Zyl, U.S. Provisional Patent Application Nos. 60/372,872 and 60/390,142).
|
| Description |
rep B Control (Cy5) vs. Pathogen (Cy3) (12780954)
|
| Data processing |
Raw intensity generated with QuantArray and statistically analysed using 2-interconnected mixed linear model approach.
|
| |
|
| Submission date |
Jul 28, 2006 |
| Last update date |
Jul 31, 2006 |
| Contact name |
Chris Smith |
| Organization name |
NCSU
|
| Department |
Bioinformatics Research Center
|
| Lab |
1500 Partners II
|
| Street address |
840 Main Campus Dr
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
| |
|
| Platform ID |
GPL4039 |
| Series (2) |
| GSE5407 |
Comparative transcript profiling of interaction events in Pinus sylvestris root tissues following challenge (15dpi). |
| GSE5410 |
Comparative transcriptome analysis of early interaction events in Scots pine root tissues following challenge |
|
