|
| Status |
Public on Jun 03, 2014 |
| Title |
iaa3 RNA-Seq replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Etiolated iaa3 seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: Etiolated seedlings
|
| Growth protocol |
Seedlings were grown on medium containing 2 µM propiconazole (PPZ) in the dark for 5 days and treated with mock or 100 nM BL for 4 hr before harvesting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using the Spectrum Plant Total RNA kit (Sigma, USA). Libraries were constructed using TruSeqTM RNA Sample Preparation Kit (Illumina) according to the manufacturer’s procedure.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
RNA-Seq of iaa3 replicate 2
|
| Data processing |
Basecalls performed using CASAVA version 1.?
Total reads were mapped to the Arabidopsis thaliana genome (TAIR9, www.arabidopsis.org) using TopHat software. Read counts corresponding genes were generated using HTSeq with union mode.
Genome_build: TAIR9
|
| |
|
| Submission date |
Oct 28, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Eunkyoo Oh |
| Organization name |
Carnegie Institution
|
| Department |
Plant Biology
|
| Street address |
260 Panama Street
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL13222 |
| Series (2) |
| GSE51772 |
Interactions of ARF6 with PIF4, BZR1, and RGA integrate auxin signaling with environmental and other hormonal signals in Arabidopsis [RNA-Seq] |
| GSE51773 |
Interactions of ARF6 with PIF4, BZR1, and RGA integrate auxin signaling with environmental and other hormonal signals in Arabidopsis |
|
| Relations |
| BioSample |
SAMN02385018 |
| SRA |
SRX368991 |
