|
Status |
Public on Jan 29, 2014 |
Title |
Patient 5 |
Sample type |
RNA |
|
|
Source name |
Urine: Extracellular Vesicles
|
Organism |
Homo sapiens |
Characteristics |
disease state: bladder cancer Sex: M age: 59 sample type: Urine Extracellular Vesicles
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA
|
Label |
biotin
|
Label protocol |
TargetAmp™ Nano-g™ Biotin-aRNA Labeling Kit for the Illumina® System, Epicentre (Cat.Num. TAN07924)
|
|
|
Hybridization protocol |
Whole-Genome Gene Expression Direct Hybridization Illumina Inc.'s protocol
|
Scan protocol |
BeadScan Illumina Inc.
|
Data processing |
Raw data were decoded by GenomeStudio Illumina Inc. And normalization was done (1) forcePositive background correction, (2) log2 transformation and (3) quantile normalization Normalized data includes data only for probes (> 4000) that gave normalized signals. In addition, the data considered in this project are RNAs present in cellular vesicles, and as such, the number of probes present in these systems is quite small.
|
|
|
Submission date |
Oct 29, 2013 |
Last update date |
Jan 29, 2014 |
Contact name |
Ana Maria Aransay |
E-mail(s) |
amaransay@cicbiogune.es
|
Phone |
0034944061325
|
Organization name |
CIC bioGUNE
|
Department |
Genome Analysis Platform
|
Street address |
Parque tecnologico de Bizkaia, Building 801-A
|
City |
Derio |
State/province |
BIZKAIA |
ZIP/Postal code |
48160 |
Country |
Spain |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE51843 |
A Pilot Study on the Potential of RNA-Associated to Urinary Vesicles as a Suitable Non-Invasive Source for Diagnostic Purposes in Bladder Cancer |
|