|
Status |
Public on Dec 18, 2013 |
Title |
A0R_d0_rep2 |
Sample type |
RNA |
|
|
Source name |
A549 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: A549 rounds of in vivo selection: 0 5-azacytidine status: untreated
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (QIAgen Hilden). RNA quality was approved using the Agilent RNA 6000 Nano Kit (Agilent Technologies).
|
Label |
biotin
|
Label protocol |
The samples were labeled according to the Affymetrix GeneChip WT Terminal Labeling protocol (Affymetrix UK Ltd., High Wycombe, UK).
|
|
|
Hybridization protocol |
Arrays were hybridized and washed according to the manufacturer's specifications (Affymetrix Inc., Santa Clara, CA, USA)
|
Scan protocol |
The arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Gene expression data from A459 cell line
|
Data processing |
Expression data was normalized, background corrected and summarized using the RMA algorithm implemented in the Affymetrix Expression Console software Build 1.3.1.187.
|
|
|
Submission date |
Nov 06, 2013 |
Last update date |
Sep 01, 2016 |
Contact name |
Christian Rohde |
E-mail(s) |
christian.rohde@uni-heidelberg.de
|
Organization name |
Heidelberg University
|
Lab |
Molecular Hematology and Oncology
|
Street address |
Im Neuenheimer Feld 410
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL6244 |
Series (2) |
GSE44390 |
DNA Methyltransferase inhibition reverses epigenetically embedded phenotypes in lung cancer preferentially affecting Polycomb target genes |
GSE52143 |
Changes in global gene expression in lung cancer cell lines A549 (A) and HTB56 (H) [Affymetrix microarrays] |
|
Relations |
Reanalyzed by |
GSE86357 |