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Sample GSM1260279 Query DataSets for GSM1260279
Status Public on Dec 18, 2013
Title A0R_d0_rep2
Sample type RNA
 
Source name A549 cell line
Organism Homo sapiens
Characteristics cell line: A549
rounds of in vivo selection: 0
5-azacytidine status: untreated
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Mini Kit (QIAgen Hilden). RNA quality was approved using the Agilent RNA 6000 Nano Kit (Agilent Technologies).
Label biotin
Label protocol The samples were labeled according to the Affymetrix GeneChip WT Terminal Labeling protocol (Affymetrix UK Ltd., High Wycombe, UK).
 
Hybridization protocol Arrays were hybridized and washed according to the manufacturer's specifications (Affymetrix Inc., Santa Clara, CA, USA)
Scan protocol The arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from A459 cell line
Data processing Expression data was normalized, background corrected and summarized using the RMA algorithm implemented in the Affymetrix Expression Console software Build 1.3.1.187.
 
Submission date Nov 06, 2013
Last update date Sep 01, 2016
Contact name Christian Rohde
E-mail(s) christian.rohde@uni-heidelberg.de
Organization name Heidelberg University
Lab Molecular Hematology and Oncology
Street address Im Neuenheimer Feld 410
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platform ID GPL6244
Series (2)
GSE44390 DNA Methyltransferase inhibition reverses epigenetically embedded phenotypes in lung cancer preferentially affecting Polycomb target genes
GSE52143 Changes in global gene expression in lung cancer cell lines A549 (A) and HTB56 (H) [Affymetrix microarrays]
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA-normalized intensities in log-2

Data table
ID_REF VALUE
7896738 2.753182
7896740 3.028292
7896742 4.850276
7896744 3.259432
7896746 9.615744
7896748 10.15598
7896750 6.102024
7896752 9.668736
7896754 4.002229
7896756 4.288933
7896759 6.380176
7896761 6.801046
7896779 6.451092
7896817 6.049767
7896822 8.249885
7896859 5.407098
7896861 3.604875
7896863 5.135876
7896865 6.78729
7896878 7.347113

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1260279_A0R_d0_rep2.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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