|
Status |
Public on Aug 23, 2006 |
Title |
PatientDD Chronic RNA_rep2 (DD117 Chronic) |
Sample type |
RNA |
|
|
Source name |
PatientDD Chronic RNA_rep2
|
Organism |
Homo sapiens |
Characteristics |
PBMC
|
Biomaterial provider |
Donor Patient six months after the initiation of IFN(Beta)1a (AVONEX, Biogen-Idec) therapy
|
Extracted molecule |
total RNA |
Extraction protocol |
PBMC total RNA is prepared by RNAeasy protocol. RNA quality checked on bioanalyzer.
|
Label |
Biotin
|
Label protocol |
The Codelink Reagents for IVT kit is used to label 2 µg of total RNA to produce UTP-biotinylated cRNA targets.
|
|
|
Hybridization protocol |
Standard CodeLink procedures
|
Scan protocol |
Standard CodeLink procedures
|
Description |
Bitynylated cRNA is probe is prepared and hybridization is carried out following the Codelink protocols and reagents
|
Data processing |
Normalized intensities analysed by by SAM using two fold cutoff.
|
|
|
Submission date |
Aug 21, 2006 |
Last update date |
Aug 22, 2006 |
Contact name |
Mandal. K. Singh |
E-mail(s) |
mandal.singh@chp.edu
|
Phone |
412-692-8402
|
Fax |
412-692-6918
|
Organization name |
Childrens Hospital of Pittsburgh
|
Department |
Transplant
|
Street address |
3460 Fifth Ave
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15206 |
Country |
USA |
|
|
Platform ID |
GPL4191 |
Series (1) |
GSE5574 |
Gene expression in PBMC from Multiple Sclerosis patients undergoing interferon beta therapy. |
|