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Status |
Public on Mar 27, 2014 |
Title |
primary tumor sample long-term OS 3081F |
Sample type |
RNA |
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|
Source name |
Human Brain, primary Glioblastoma
|
Organism |
Homo sapiens |
Characteristics |
survival: long-term OS tissue: Human Brain, primary Glioblastoma
|
Treatment protocol |
Tumor samples were shock-frozen after resection and stored at -80 °C. Only specimens with a histologically estimated tumor cell content of 80% or more were used for molecular analyses.
|
Extracted molecule |
total RNA |
Extraction protocol |
DNA and RNA were extracted by ultracentrifugation.
|
Label |
biotin
|
Label protocol |
Sample preparation was done with 2.5 μg total-RNA using the One Cycle Target Labelling and Controls kit (Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Gene expression data from primary Glioblastoma
|
Data processing |
Raw probe intensities were calibrated and summarized into one expression value per probe set using the hook method, and then quantile-normalised. The expression value of each gene was transformed into log10-scale and centered with respect to its mean value averaged over all samples investigated
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Submission date |
Dec 30, 2013 |
Last update date |
Mar 28, 2014 |
Contact name |
Edith Willscher |
E-mail(s) |
willscher@izbi.uni-leipzig.de
|
Organization name |
IZBI
|
Street address |
Haertelstraße 16-18
|
City |
Leipzig |
ZIP/Postal code |
04107 |
Country |
Germany |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE53733 |
Expression data from primary Glioblastoma in adults |
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