NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1304726 Query DataSets for GSM1304726
Status Public on Jul 02, 2014
Title FC
Sample type SRA
 
Source name Microdissected lens central fibers
Organism Gallus gallus
Characteristics age: Embryonic day 13
tissue: Lens
tissue (differentiation state-specific region): central fibers
#pooled microdissections: 100
Extracted molecule total RNA
Extraction protocol The following protocol was performed in replicate. 100 Lenses were removed, microdissected (lens central epithelium, equatorial epithelium, cortical fibers, and central fiber core), pooled, and RNA was harvested using Trizol reagent. illumina directional mRNA-Seq Sample Preparation was performed under manufacturer guidelines for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
 
Description Sample 7
Data processing Prepared libraries were sequenced uni-directionally with the illumina Genome Analyzer IIx.
Reads aligned to ENSEMBLE; Galgal4; GCA_000002315.2.
Data was then processed using the cufflinks pipeline with replicates under default parameters. (http://cufflinks.cbcb.umd.edu/)
Differential expression analysis performed by cuffdiff version 2.1.1
Genome_build: Gallus_gallus-4.0 (Synonym: galGal4)
Supplementary_files_format_and_content: Tab-delimited text files including FPKM values and/or statistical analysis for the final assembled dataset
 
Submission date Jan 10, 2014
Last update date May 15, 2019
Contact name Marc Kantorow
E-mail(s) mkantoro@fau.edu
Phone 5612973806
Organization name Florida Atlantic University
Street address 777 Glades Rd
City Boca Raton
State/province Florida
ZIP/Postal code 33431
Country USA
 
Platform ID GPL13797
Series (1)
GSE53976 Differentiation state-specific mitochondrial dynamic regulatory networks are revealed by global transcriptional analysis of the developing chicken lens.
Relations
BioSample SAMN02581119
SRA SRX424601

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap