Fluorescent-labeled DNA (Cy3) was produced through Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described in detail (Guo, Y. et al. J. Virol. 79, 14392-14403, 2005). Briefly, double-stranded cDNA containing T7 RNA polymerase promoter sequence was synthesized with 5 ug of total RNA using Reverse Transcription System, RNase H, DNA polymerase I and T4 DNA polymerase, according to the manufacturer’s recommended protocol (Promega).
Scan protocol
Slides were scanned with a confocal LuxScan scanner (CapitalBio Corp.). The data from the obtained images were extracted with SpotData software (CapitalBio Corp.).
Description
NA
Data processing
The data from the obtained images were extracted with SpotData software (CapitalBio Corp.). Median-scaling of background-subtracted intensity was applied to each array to make median equal to 1000.
