|
| Status |
Public on Feb 06, 2014 |
| Title |
Neg B |
| Sample type |
RNA |
| |
|
| Source name |
Splenocytes- non CAR T-cells and MDSCs
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
strain: Balb/c
tissue: Spleen
sorted cells: negative fraction
|
| Treatment protocol |
Flow cytometric Cell Sorting from dissociated murine spleens
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Mini Kit
|
| Label |
FAM
|
| Label protocol |
cDNA was generated from 1 microgram of RNA using the Life Technologies RNA-cDNA kit (manufacturers protocol). Real-time PCR was performed on a 7900HT machine using the standard TLDA protcol.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
CD4-CD34-CD11b-Gr-1-
|
| Data processing |
The real-time PCR data was analysed using SDS2.4 software according to the manufacturers protocol with automatic baseline and threshold calling
Relative expression data was genrated by comparing each amplicon to the GEOMEAN of the samples. Amplicons with no signal for any sample were removed.
Relative expression data (log2) was transferred to MeV software and used to generate heatmaps and hierarchical clustering.
|
| |
|
| Submission date |
Feb 05, 2014 |
| Last update date |
Feb 06, 2014 |
| Contact name |
Dominic Graham Rothwell |
| E-mail(s) |
drothwell@picr.man.ac.uk
|
| Phone |
01614463202
|
| Organization name |
University of Manchester
|
| Department |
CEP
|
| Lab |
Nucleic Acid Biomarkers
|
| Street address |
Wilmslow Road
|
| City |
Manchester |
| State/province |
Manchester |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL18252 |
| Series (1) |
| GSE54696 |
Production of cytokines by CD4+CAR+ T-cells in spleens |
|
