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Sample GSM1335195 Query DataSets for GSM1335195
Status Public on Feb 27, 2014
Title Mouse round spermatids, biological rep 2, chip B
Sample type RNA
 
Source name Mouse round spermatids
Organism Mus musculus
Characteristics strain: Balb/c
gender: male
age: 60 days
cell type: round spermatids
Treatment protocol Testes from mice at different ages were harvested and decapsulated. Isolated seminiferous tubules were enzymatic digested and mechanically dissociated to single cells, and undergone differential plating for 2 hours to eliminate Sertoli cells. Cells at different developmental stages were isolated by a preivously described method (Bellvé AR, et al. J Cell Biol 1977; 74:68-85). The purities of three isolated cell types were all above 90%.
Growth protocol Balb/c mice were on a 12-hour light-dark cycle in a pathogen-free animal facility.
Extracted molecule total RNA
Extraction protocol 15 micrograms of total RNA was extracted for each biological replicate. RNA quality was validated with Bioanalyzer 2100 (Agilent). mRNA was enriched with RiboMinus Human/Mouse Transcriptome Isolation Kit according to manufacturer's protocol.
Label Biotin
Label protocol cDNA was synthesized with GeneChip WT Amplified Double-Stranded cDNA Synthesis Kit and amplified with GeneChip WT cDNA Amplification Kit. Amplified double-stranded cDNA was labeled with GeneChip WT Double-Strand DNA Terminal Labeling Kit according to manufacturer's guideline.
 
Hybridization protocol Purified biotin-labelled cDNA was hybrided to the microarray gene for 16 hours at 45C with 60 rpm rotation on Affymetrix Mouse Tiling 1.1R Array Set (14 GeneChips per set). GeneChips were washed and stained in the Afymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G and the images were obtained by the Affymetrix GeneChip Operating Software (GCOS).
Description Sptd_B.bar
Affymetrix Mouse Tiling 1.1R Array Set
Data processing Expression data were analyzed with Affymetrix Tiling Analysis Software (TAS) and library files BPMAP files version 35 (available from Affymetrix website http://www.affymetrix.com/)
A BAR file was created for each triplicate.
The resulting BAR files were generated by the Affymetrix TAS using probe analysis parameters: Bandwidth = 75, Test type = One side Upper, Intensities = PM/MM
 
Submission date Feb 26, 2014
Last update date Feb 27, 2014
Contact name Tin-Lap Lee
Phone +852 39434436
Organization name The Chinese University of Hong Kong
Department School of Biomedical Sciences
Street address Area 39
City Shatin
ZIP/Postal code 00000
Country Hong Kong
 
Platform ID GPL13195
Series (1)
GSE55381 Whole transcription data for mouse spermatogonial cells

Supplementary file Size Download File type/resource
GSM1335195_Sptd-2B.CEL.gz 26.8 Mb (ftp)(http) CEL
Processed data are available on Series record

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