|
| Status |
Public on Jun 20, 2017 |
| Title |
glp-4_PA14_SK_A |
| Sample type |
RNA |
| |
|
| Source name |
glp-4,PA14, 8h, SK agar
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
media: SK, PMID: 9892699
treatment: P. aeruginosa PA14
tissue: Whole organism
|
| Treatment protocol |
Animals were grown until young adult stage. Afterward, worms were incubated for 8 hours at 25°C on 10 cm plates with solid agar or in 6-well plates with liquid media with appropriate bacterial strain and/or chemical.
|
| Growth protocol |
glp-4(bn2ts) worms were synchronized by hypochlorite treatment. Arrested L1s were plated on 10 cm NGM plates seeded with E. coli OP50 and grown at 15°C overnight then shifted to 25°C for 44h until they reached sterile young adult stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRI Reagent (Molecular Research Center) according to manufacturer's instructions and purified using an RNeasy column (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Data processing |
Data were analyzed with GC-RMA in the R programming language, using the Bioconductor package gcrma. Analysis was performed using default settings.
|
| |
|
| Submission date |
Feb 27, 2014 |
| Last update date |
Jun 22, 2017 |
| Contact name |
Natalia V Kirienko |
| E-mail(s) |
kirienko@rice.edu
|
| Organization name |
Rice University
|
| Department |
BioSciences
|
| Lab |
Kirienko
|
| Street address |
6100 Main St. MS140
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77005 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE55422 |
A Conserved Mitochondrial Surveillance Pathway Is Required for Defense against Pseudomonas aeruginosa |
|
