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Status |
Public on Jun 01, 2015 |
Title |
WT in the dark at 60min (1st) |
Sample type |
RNA |
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Source name |
Synechococcus wild type cells incubated for 60 min in the dark after the start point (hour 12 under continuous light after the two LD cycles)
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Organism |
Synechococcus elongatus PCC 7942 = FACHB-805 |
Characteristics |
protocol: Dark_60min
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Treatment protocol |
Cells were collected at indicated times and stored at -80C until use
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Growth protocol |
Synechococcus cells were grown in continuous culture systems in any conditions at 30C under the light (40 micromol/m^2/sec)
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted with a modified hot-phenol extraction method
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Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix prokaryotic protocol from 10 ug total RNA (Expression Analysis Technical Manual)
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Hybridization protocol |
Following fragmentation, 1.5 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Synechococcus Custom Arrays (Syel6301a520114F). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
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Description |
Gene expression data from Synechococcus elongatus PCC 7942
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Data processing |
The data were analyzed with GeneChip Operating Software (GCOS) ver 1.2.1.001 using Affymetrix default analysis settings to obtain raw data.
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Submission date |
Mar 06, 2014 |
Last update date |
Jun 01, 2015 |
Contact name |
Hideo Iwasaki |
E-mail(s) |
hideo-iwasaki@waseda.jp
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Organization name |
Waseda University
|
Department |
Electrical Engineering & Bioscience
|
Street address |
TWIns 1F 2-2 Wakamatsu-cho, Shinjuku
|
City |
Tokyo |
ZIP/Postal code |
162-8480 |
Country |
Japan |
|
|
Platform ID |
GPL8012 |
Series (1) |
GSE55637 |
Transcriptional profile of Synechococcus in the dark or light with or without photosynthesis inhibitors |
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