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Sample GSM1340665 Query DataSets for GSM1340665
Status Public on Jun 01, 2015
Title WT in the light+DCMU at 60min (1st)
Sample type RNA
 
Source name Synechococcus wild type cells incubated for 60 min in the light with DCMU after the start point (hour 12 under continuous light after the two LD cycles)
Organism Synechococcus elongatus PCC 7942 = FACHB-805
Characteristics protocol: Light+DCMU_60min
Treatment protocol Cells were collected at indicated times and stored at -80C until use
Growth protocol Synechococcus cells were grown in continuous culture systems in any conditions at 30C under the light (40 micromol/m^2/sec)
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted with a modified hot-phenol extraction method
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix prokaryotic protocol from 10 ug total RNA (Expression Analysis Technical Manual)
 
Hybridization protocol Following fragmentation, 1.5 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Synechococcus Custom Arrays (Syel6301a520114F). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Gene expression data from Synechococcus elongatus PCC 7942
Data processing The data were analyzed with GeneChip Operating Software (GCOS) ver 1.2.1.001 using Affymetrix default analysis settings to obtain raw data.
 
Submission date Mar 06, 2014
Last update date Jun 01, 2015
Contact name Hideo Iwasaki
E-mail(s) hideo-iwasaki@waseda.jp
Organization name Waseda University
Department Electrical Engineering & Bioscience
Street address TWIns 1F 2-2 Wakamatsu-cho, Shinjuku
City Tokyo
ZIP/Postal code 162-8480
Country Japan
 
Platform ID GPL8012
Series (1)
GSE55637 Transcriptional profile of Synechococcus in the dark or light with or without photosynthesis inhibitors

Data table header descriptions
ID_REF
VALUE GCOS intensity normalized as described above (data processing)

Data table
ID_REF VALUE
syc0001-h_at 79.8
syc0002-h_at 193.5
syc0003-m_at 372.2
syc0004-m_at 159.9
syc0005-m_at 109.6
syc0006-h_at 152.6
syc0007-m_at 103.5
syc0008-m_at 198.3
syc0009-m_at 293.3
syc0010-h_at 554.7
syc0011-m_at 96.4
syc0012-h_at 2095.8
syc0013-m_at 1031.7
syc0014-m_at 634.4
syc0015-h_at 3016.6
syc0016-m_at 89.2
syc0017-m_at 136.9
syc0018-m_at 86.7
syc0019-h_at 1175.8
syc0020-h_at 557

Total number of rows: 2515

Table truncated, full table size 45 Kbytes.




Supplementary file Size Download File type/resource
GSM1340665_WT_Light+DCMU_60min_1.CEL.gz 939.5 Kb (ftp)(http) CEL
Processed data included within Sample table

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