|
| Status |
Public on Mar 01, 2015 |
| Title |
BE2C_shMEIS2_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
BE(2)-C/pLKO.1-shMEIS2-43 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BE(2)-C
treatment: MEIS2 depletion
|
| Treatment protocol |
None
|
| Growth protocol |
BE(2)-C cells were infected with pLKO.1-based lentiviruses expressing either shGFP or shMEIS2-43 for 48 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the cell samples using Trizol (Invitrogen), and RNA quality and quantity was assessed using a Agilent BioAnalyzer and a Nanodrop Spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
TdT-labled cRNA were prepared with an Affymetrix GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Description |
RNA expression value
|
| Data processing |
Data were normalized, significance determined by ANOVA, and fold change calculated with the Partek Genomics Suite (Partek Inc., St. Louis, MO). Gene annotation enrichment analysis was performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) v6.7 for all significantly changed genes (≥ ±1.5 fold, P < 0.01) and Gene Set Enrichment Analysis (GSEA).
|
| |
|
| Submission date |
Mar 18, 2014 |
| Last update date |
Mar 01, 2015 |
| Contact name |
Justin Choi |
| E-mail(s) |
jeochoi@gmail.com
|
| Phone |
1-706-721-6757
|
| Organization name |
Georgia Regents University
|
| Department |
Cancer Center
|
| Street address |
1410 Laney Walker Blvd.
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE56003 |
Gene expression profiling of human neuroblastoma BE(2)-C cells with MEIS2 depletion |
|
