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Sample GSM1356646 Query DataSets for GSM1356646
Status Public on Mar 25, 2014
Title AzaLCA1
Sample type RNA
 
Source name left carotid artery, 21 days of 5-Aza-2'deoxycytidine treatment (0.2mg/kg/day by I.P. injection), 7 days ligated
Organism Mus musculus
Characteristics treatment: 5-Aza-2'deoxycytidine treatment, 7 days ligated
strain: C57Bl6
age: 9 weeks
tissue: carotid artery intima
Treatment protocol Mice were treated with either saline control or 5-Aza-2'deoxycytidine (0.2mg/kg/day) by daily I.P. injection for 3 weeks. After 2 weeks of treament, 3 out of the 4 caudal branches of the left carotid artery (LCA) of mice were ligated while the right carotid artery (RCA) was left untouched as the contralateral internal control. After 1 week of ligation, mice were sacrificed by CO2 inhalation.
Growth protocol C57Bl6 mice were fed normal chow diet for 9 weeks.
Extracted molecule total RNA
Extraction protocol Mice were perfused with saline and carotid arteries were extracted and flushed with 150 ul of Qiazol. The Qiagen miReasy Kit was used to extract and purify total RNA. Quality control was performed with Agilent Bioanalyzer.
Label biotin
Label protocol PCR product was prepared with standard Illumina protocol for arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol BeadChips were scanned using Illumina HiScan.
Description endothelial-enriched RNA from carotid artery of C57Bl6 male 9 weeks of age
endothelial-enriched RNA (3 pooled arteries),mouse left carotid artery, 7 days ligated, 21 days of 5-Aza-2'deoxycytidine treatment (0.2mg/kg/day by I.P. injection), replicate #1
Data processing The data were normalized using quantile-normalization with GenomeStudio V2010.3
 
Submission date Mar 24, 2014
Last update date Mar 25, 2014
Contact name Jessilyn Dunn
E-mail(s) jpdunn@emory.edu
Phone 404-727-3223
Organization name Georgia Tech & Emory University
Department Biomedical Engineering
Lab Hanjoong Jp
Street address 1760 Haygood Drive HSRB E197
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platform ID GPL6887
Series (1)
GSE56143 Identification of mechano- and/or 5Aza-sensitive genes in mouse carotid artery endothelium in vivo exposed to disturbed blood flow for 7 days

Data table header descriptions
ID_REF
VALUE The values are linear and represent quantile-normalized expression signal
AzaLCA1.Detection Pval

Data table
ID_REF VALUE AzaLCA1.Detection Pval
ILMN_2417611 83.46237 0.2596154
ILMN_2762289 82.81503 0.2970085
ILMN_2896528 196.6552 0
ILMN_2721178 120.75 0.001068376
ILMN_2458837 78.52727 0.7788461
ILMN_3033922 314.0288 0
ILMN_3092673 689.2065 0
ILMN_1230777 477.4283 0
ILMN_1246069 91.2028 0.0224359
ILMN_1232042 81.13583 0.5032051
ILMN_1243193 169.698 0
ILMN_2524361 76.4295 0.9423077
ILMN_1242440 82.36594 0.3450855
ILMN_1233188 148.7983 0
ILMN_2543688 374.5647 0
ILMN_1259789 87.16899 0.08012821
ILMN_2816356 80.73422 0.5438034
ILMN_1224596 104.0912 0.004273504
ILMN_1233643 98.71879 0.006410256
ILMN_2808939 113.4814 0.003205128

Total number of rows: 45281

Table truncated, full table size 1354 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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