|
| Status |
Public on Mar 26, 2014 |
| Title |
Treated/untreated cell 1, replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
dox-treated
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain/background: R1158
genotype/variation: TETp-HSC82 hsp82Δ arg4Δ lys5Δ car2Δ::URA3
treatment: doxycycline
|
| Treatment protocol |
For biological replicate 1, light-labeled cells were treated with 5 μg/mL dox for 11 hours until log phase. For biological replicate 2, heavy-labeled cells were treated, instead.
|
| Growth protocol |
Cells were grown to log phase in a modified synthetic complete (SC) medium which contains double the amount of amino acid supplements containing either 40 mg/L L-arginine and 60 mg/L L-lysine (light labeling) or 40 mg/L 13C6-arginine and 60 mg/L 13C6-lysine (heavy labeling).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy Midi Kit (Qiagen, Valencia, CA).
|
| Label |
Alexa Fluor 647
|
| Label protocol |
Labeling protocol was adapted from Microarray Core Facility, Institute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html).
|
| |
|
| Channel 2 |
| Source name |
untreated
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain/background: R1158
genotype/variation: TETp-HSC82 hsp82Δ arg4Δ lys5Δ car2Δ::URA3
treatment: none
|
| Treatment protocol |
For biological replicate 1, light-labeled cells were treated with 5 μg/mL dox for 11 hours until log phase. For biological replicate 2, heavy-labeled cells were treated, instead.
|
| Growth protocol |
Cells were grown to log phase in a modified synthetic complete (SC) medium which contains double the amount of amino acid supplements containing either 40 mg/L L-arginine and 60 mg/L L-lysine (light labeling) or 40 mg/L 13C6-arginine and 60 mg/L 13C6-lysine (heavy labeling).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy Midi Kit (Qiagen, Valencia, CA).
|
| Label |
Alexa Fluor 555
|
| Label protocol |
Labeling protocol was adapted from Microarray Core Facility, Institute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html).
|
| |
|
| |
| Hybridization protocol |
Hybridization protocol was adapted from Microarray Core Facility, Institute of Molecular Biology, Academia Sinica, TW (http://www.imb.sinica.edu.tw/mdarray/methods.html).
|
| Scan protocol |
Arrays were scanned for F635 and F532 fluorescence intensities using AXON 4000B (Molecular Device, Sunnyvale, CA).
|
| Description |
dox1-2
Biological replicate 1 of 2. Technical replicate 2 of 3. Dox-treated/untreated log-phase cells.
|
| Data processing |
LOWESS normalized, background subtracted data obtained from signals. Agilent GeneSpring GX 7.3 was used.
|
| |
|
| Submission date |
Mar 25, 2014 |
| Last update date |
Mar 28, 2014 |
| Contact name |
Jun-Yi Leu |
| E-mail(s) |
jyl9216public@gmail.com
|
| Organization name |
Institute of Molecular Biology
|
| Lab |
N411
|
| Street address |
No. 128, Sec. 2, Academia Rd., Nangang Dist.
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL18498 |
| Series (1) |
| GSE56186 |
Saccharomyces cerevisiae mutant strain with R1158 strain background: Fold change after doxycycline treatment |
|
