|
Status |
Public on Oct 01, 2006 |
Title |
MamGland_WildType_Virgin_replicate3 |
Sample type |
RNA |
|
|
Source name |
Mammary Gland
|
Organism |
Mus musculus |
Characteristics |
Virgin whole mammary gland No4 (inguinal) harvested from C57BL/6J female mouse at puberty (6 wks of age).
|
Treatment protocol |
Excised tissue was placed in RNAlater (Ambion Inc) and kept at –80oC. RNA was isolated using Trizol (GibcoBRL).
|
Extracted molecule |
total RNA |
Label |
ENZO biotin label
|
Label protocol |
Approximately 10 micrograms of total RNA was processed to produce biotinylated cRNA targets. Fragmented cRNA was prepared (10 micrograms) and hybridized overnight to MG-U74AV2 chip.
|
|
|
Hybridization protocol |
Standard Affymetrix procedure
|
Scan protocol |
Standard Affymetrix procedure
|
Description |
Wild type (C57BL/6J), Virgin whole mammary gland No4
|
Data processing |
MAS5.0
|
|
|
Submission date |
Sep 12, 2006 |
Last update date |
Sep 14, 2006 |
Contact name |
Juan F. Medrano |
E-mail(s) |
jfmedrano@ucdavis.edu
|
Organization name |
University of California
|
Department |
Animal Science
|
Lab |
Animal Genomics-Medrano
|
Street address |
One Shields Ave
|
City |
Davis |
State/province |
CA |
ZIP/Postal code |
95616-8521 |
Country |
USA |
|
|
Platform ID |
GPL81 |
Series (1) |
GSE5831 |
Analysis of the mammary gland transcriptome in wild type C57BL/6J mice |
|