NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM135899 Query DataSets for GSM135899
Status Public on Oct 01, 2006
Title MamGland_WildType_Pregnant_replicate2
Sample type RNA
 
Source name Mammary Gland
Organism Mus musculus
Characteristics Whole mammary gland No4 (inguinal) harvested from C57BL/6J female mouse at 14 days of pregnancy.
Treatment protocol Excised tissue was placed in RNAlater (Ambion Inc) and kept at –80oC. RNA was isolated using Trizol (GibcoBRL).
Extracted molecule total RNA
Label ENZO biotin label
Label protocol Approximately 10 micrograms of total RNA was processed to produce biotinylated cRNA targets. Fragmented cRNA was prepared (10 micrograms) and hybridized overnight to MG-U74AV2 chip.
 
Hybridization protocol Standard Affymetrix procedure
Scan protocol Standard Affymetrix procedure
Description Wild type (C57BL/6J), whole mammary gland No4 from 14 day pregnant females.
Data processing MAS5.0
 
Submission date Sep 12, 2006
Last update date Sep 14, 2006
Contact name Juan F. Medrano
E-mail(s) jfmedrano@ucdavis.edu
Organization name University of California
Department Animal Science
Lab Animal Genomics-Medrano
Street address One Shields Ave
City Davis
State/province CA
ZIP/Postal code 95616-8521
Country USA
 
Platform ID GPL81
Series (1)
GSE5831 Analysis of the mammary gland transcriptome in wild type C57BL/6J mice

Data table header descriptions
ID_REF
VALUE Intensity
ABS_CALL Present, Absent, or Marginal

Data table
ID_REF VALUE ABS_CALL
AFFX-MurIL2_at 6.3 A
AFFX-MurIL10_at 17.2 A
AFFX-MurIL4_at 9.6 A
AFFX-MurFAS_at 30.3 A
AFFX-BioB-5_at 113.8 P
AFFX-BioB-M_at 258.8 P
AFFX-BioB-3_at 175.3 P
AFFX-BioC-5_at 463 P
AFFX-BioC-3_at 323.6 P
AFFX-BioDn-5_at 500.5 P
AFFX-BioDn-3_at 2146.6 P
AFFX-CreX-5_at 4169.4 P
AFFX-CreX-3_at 6418 P
AFFX-BioB-5_st 17.1 A
AFFX-BioB-M_st 14.1 A
AFFX-BioB-3_st 7.6 A
AFFX-BioC-5_st 9.5 A
AFFX-BioC-3_st 3.3 A
AFFX-BioDn-5_st 31.6 A
AFFX-BioDn-3_st 43.9 A

Total number of rows: 12488

Table truncated, full table size 207 Kbytes.




Supplementary file Size Download File type/resource
GSM135899.CEL.gz 2.7 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap