|
| Status |
Public on Dec 31, 2006 |
| Title |
B6J embryo, alcohol & PK11195-6J-CoT-1 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic headfold
|
| Organism |
Mus musculus |
| Characteristics |
8 days post-coitum, 4-8 somite pair stage
|
| Biomaterial provider |
cranial neural folds (headfold) pooled from 1 litter
|
| Treatment protocol |
2.9g/kg EtOH + 4.0 mg/kg PK11195
|
| Growth protocol |
3.0h after maternal treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA isolated with RNeasy (Qiagen) that removes small RNAs (<200 nucleotides); RNA passing QA/QC amplified with Agilent Low RNA Input Fluorescent Linear Amplification Kit labeling to cRNA.
|
| Label |
biotinylated nucleotide analogue
|
| Label protocol |
streptavidin-conjugated peroxidase and cyanine-3
|
| |
|
| Hybridization protocol |
16h, 45°C, Affymetrix Mouse Genome 430A Array
|
| Scan protocol |
intensity, GeneChip 3000 scanner
|
| Description |
PK11195 counterexposure (Cy3), B6J headfold
|
| Data processing |
Probe-level data normalization used the robust multichip average (RMA). Data were analyzed by fitting to the linear model and computing the coefficients for each element and sample pair average (less control elements).
|
| |
|
| Submission date |
Sep 13, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Thomas B Knudsen |
| E-mail(s) |
singh.amarv@epamail.epa.gov
|
| Phone |
502-852-4128
|
| Fax |
502-852-4702
|
| URL |
http://systemsanalysis.louisville.edu/
|
| Organization name |
University of Louisville
|
| Department |
Birth Defects Center
|
| Lab |
Systems Analysis Laboratory
|
| Street address |
501 S Preston St
|
| City |
Louisville |
| State/province |
KY |
| ZIP/Postal code |
40202 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE1074 |
Strain-dependent effects of alcohol on early mouse embryos |
|
| Relations |
| Reanalyzed by |
GSE119085 |
