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Sample GSM1365690 Query DataSets for GSM1365690
Status Public on Sep 01, 2015
Title COG_010
Sample type RNA
 
Source name PBMC, 60min post-OGTT
Organism Homo sapiens
Characteristics age: 18
gender: M
bmi: 23.35
Treatment protocol Participants consumed 75 g glucose (anhydrous) in 150 mL of water. PBMCs were collected at baseline and 60 minutes post-challenge
Growth protocol NA
Extracted molecule total RNA
Extraction protocol cDNA was synthesized from total PBMC RNA using the Ambion WT Expression kit (Applied Biosystems, CA).
Label biotin
Label protocol Synthesized cDNA was labeled using the GeneChip® WT Terminal Labeling and Hybridization kit (AffymetrixInc, Santa Clara, CA).
 
Hybridization protocol Labeled cDNA was then hybridized to human whole-genome Gene ST 1.0 arrays encoding 28 869 genes (AffymetrixInc). 
Scan protocol Sample labeling, hybridization to chips, and image scanning were performed according to the manufacturer's GeneChip Expression Analysis Technical Manual (AffymetrixInc), using an Affymetrix Genechip Scanner 3000
Description PBMC, 60min post-OGTT
Data processing Data were normalized by RMA. All data processing and analysis were carried out in R.
 
Submission date Apr 08, 2014
Last update date Sep 01, 2015
Contact name Melissa J Morine
E-mail(s) mmorine@gmail.com
Organization name The Microsoft Research - University of Trento Centre for Computational and Systems Biology
Street address Via Manifattura 1
City Rovereto
ZIP/Postal code 38068
Country Italy
 
Platform ID GPL6244
Series (2)
GSE56607 Peripheral blood mononuclear cell gene expression pre-/post- oral glucose challenge
GSE56609 Peripheral blood mononuclear cell gene expression pre-/post- acute metabolic challenge

Data table header descriptions
ID_REF
VALUE RMA-normalized data.

Data table
ID_REF VALUE
7892501 4.699775456
7892502 6.102577404
7892503 3.897368267
7892504 9.103457012
7892505 4.136393249
7892506 7.484046187
7892507 5.394288392
7892508 7.268380153
7892509 11.65488302
7892510 6.389311496
7892511 4.73349859
7892512 7.591468908
7892513 5.391067713
7892514 10.96359748
7892515 10.11181304
7892516 3.957771009
7892517 6.757877392
7892518 4.11984117
7892519 6.721723922
7892520 10.32375509

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM1365690_COG_010.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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