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Sample GSM1365691 Query DataSets for GSM1365691
Status Public on Sep 01, 2015
Title COG_013
Sample type RNA
 
Source name PBMC, fasting
Organism Homo sapiens
Characteristics age: 30
gender: M
bmi: 23.09
Treatment protocol Participants consumed 75 g glucose (anhydrous) in 150 mL of water. PBMCs were collected at baseline and 60 minutes post-challenge
Growth protocol NA
Extracted molecule total RNA
Extraction protocol cDNA was synthesized from total PBMC RNA using the Ambion WT Expression kit (Applied Biosystems, CA).
Label biotin
Label protocol Synthesized cDNA was labeled using the GeneChip® WT Terminal Labeling and Hybridization kit (AffymetrixInc, Santa Clara, CA).
 
Hybridization protocol Labeled cDNA was then hybridized to human whole-genome Gene ST 1.0 arrays encoding 28 869 genes (AffymetrixInc). 
Scan protocol Sample labeling, hybridization to chips, and image scanning were performed according to the manufacturer's GeneChip Expression Analysis Technical Manual (AffymetrixInc), using an Affymetrix Genechip Scanner 3000
Description PBMC, fasting
Data processing Data were normalized by RMA. All data processing and analysis were carried out in R.
 
Submission date Apr 08, 2014
Last update date Sep 01, 2015
Contact name Melissa J Morine
E-mail(s) mmorine@gmail.com
Organization name The Microsoft Research - University of Trento Centre for Computational and Systems Biology
Street address Via Manifattura 1
City Rovereto
ZIP/Postal code 38068
Country Italy
 
Platform ID GPL6244
Series (2)
GSE56607 Peripheral blood mononuclear cell gene expression pre-/post- oral glucose challenge
GSE56609 Peripheral blood mononuclear cell gene expression pre-/post- acute metabolic challenge

Data table header descriptions
ID_REF
VALUE RMA-normalized data.

Data table
ID_REF VALUE
7892501 4.363808517
7892502 6.950664777
7892503 4.864043956
7892504 9.398568636
7892505 5.653346413
7892506 9.423669778
7892507 5.256844921
7892508 8.078663023
7892509 11.17669667
7892510 6.221985185
7892511 4.549925638
7892512 8.781910807
7892513 6.225820469
7892514 9.923829459
7892515 10.05453286
7892516 3.265184098
7892517 6.881700489
7892518 4.250300565
7892519 7.097254339
7892520 10.85062265

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM1365691_COG_013.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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