|
Status |
Public on Sep 01, 2015 |
Title |
COG_017 |
Sample type |
RNA |
|
|
Source name |
PBMC, fasting
|
Organism |
Homo sapiens |
Characteristics |
age: 24 gender: M bmi: 22.37
|
Treatment protocol |
Participants consumed 75 g glucose (anhydrous) in 150 mL of water. PBMCs were collected at baseline and 60 minutes post-challenge
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
cDNA was synthesized from total PBMC RNA using the Ambion WT Expression kit (Applied Biosystems, CA).
|
Label |
biotin
|
Label protocol |
Synthesized cDNA was labeled using the GeneChip® WT Terminal Labeling and Hybridization kit (AffymetrixInc, Santa Clara, CA).
|
|
|
Hybridization protocol |
Labeled cDNA was then hybridized to human whole-genome Gene ST 1.0 arrays encoding 28 869 genes (AffymetrixInc).
|
Scan protocol |
Sample labeling, hybridization to chips, and image scanning were performed according to the manufacturer's GeneChip Expression Analysis Technical Manual (AffymetrixInc), using an Affymetrix Genechip Scanner 3000
|
Description |
PBMC, fasting
|
Data processing |
Data were normalized by RMA. All data processing and analysis were carried out in R.
|
|
|
Submission date |
Apr 08, 2014 |
Last update date |
Sep 01, 2015 |
Contact name |
Melissa J Morine |
E-mail(s) |
mmorine@gmail.com
|
Organization name |
The Microsoft Research - University of Trento Centre for Computational and Systems Biology
|
Street address |
Via Manifattura 1
|
City |
Rovereto |
ZIP/Postal code |
38068 |
Country |
Italy |
|
|
Platform ID |
GPL6244 |
Series (2) |
GSE56607 |
Peripheral blood mononuclear cell gene expression pre-/post- oral glucose challenge |
GSE56609 |
Peripheral blood mononuclear cell gene expression pre-/post- acute metabolic challenge |
|