NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1365693 Query DataSets for GSM1365693
Status Public on Sep 01, 2015
Title COG_017
Sample type RNA
 
Source name PBMC, fasting
Organism Homo sapiens
Characteristics age: 24
gender: M
bmi: 22.37
Treatment protocol Participants consumed 75 g glucose (anhydrous) in 150 mL of water. PBMCs were collected at baseline and 60 minutes post-challenge
Growth protocol NA
Extracted molecule total RNA
Extraction protocol cDNA was synthesized from total PBMC RNA using the Ambion WT Expression kit (Applied Biosystems, CA).
Label biotin
Label protocol Synthesized cDNA was labeled using the GeneChip® WT Terminal Labeling and Hybridization kit (AffymetrixInc, Santa Clara, CA).
 
Hybridization protocol Labeled cDNA was then hybridized to human whole-genome Gene ST 1.0 arrays encoding 28 869 genes (AffymetrixInc). 
Scan protocol Sample labeling, hybridization to chips, and image scanning were performed according to the manufacturer's GeneChip Expression Analysis Technical Manual (AffymetrixInc), using an Affymetrix Genechip Scanner 3000
Description PBMC, fasting
Data processing Data were normalized by RMA. All data processing and analysis were carried out in R.
 
Submission date Apr 08, 2014
Last update date Sep 01, 2015
Contact name Melissa J Morine
E-mail(s) mmorine@gmail.com
Organization name The Microsoft Research - University of Trento Centre for Computational and Systems Biology
Street address Via Manifattura 1
City Rovereto
ZIP/Postal code 38068
Country Italy
 
Platform ID GPL6244
Series (2)
GSE56607 Peripheral blood mononuclear cell gene expression pre-/post- oral glucose challenge
GSE56609 Peripheral blood mononuclear cell gene expression pre-/post- acute metabolic challenge

Data table header descriptions
ID_REF
VALUE RMA-normalized data.

Data table
ID_REF VALUE
7892501 5.877625496
7892502 7.990634193
7892503 4.330479684
7892504 8.925120768
7892505 5.246830022
7892506 8.789527395
7892507 5.934871186
7892508 8.210301986
7892509 11.47123489
7892510 6.922632876
7892511 4.597035772
7892512 7.816975095
7892513 6.389324087
7892514 11.21209019
7892515 10.13842783
7892516 3.940937055
7892517 8.160143615
7892518 4.204911361
7892519 6.964809928
7892520 10.35578906

Total number of rows: 33297

Table truncated, full table size 646 Kbytes.




Supplementary file Size Download File type/resource
GSM1365693_COG_017.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap