|
| Status |
Public on Jul 01, 2008 |
| Title |
A2lox.mesp1 (day3 + DKK1 + dox) |
| Sample type |
RNA |
| |
|
| Source name |
A2lox.mesp1 ES cells
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: Mesp1 transgenic ES cells
|
| Treatment protocol |
ES cells were differentiated in low density suspension culture. Recombinant DKK1 at 150 ng/mL was added from day 0-4 and doxycycline (250 ng/mL) was added from days 2-4.
|
| Growth protocol |
For differentiation, ES cells were plated in suspension in bacterial petri dishes at 1.5 x 10e4 cells/mL in serum-containing medium (SCM: IMDM with 10% FCS, NEAA, L-glutamine, NaPyruvate, Pen/Strep, and 2-mercaptoethanol) ), and supplemented where indicated with Dkk1 or doxycycline.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using Rneasy mini kits (Qiagen) per manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Description |
Gene expression data from ES cells differentiated in serum containing medium supplemented with DKK1 from days 0-4 and Mesp1 induction from days 2-4.
|
| Data processing |
The data were normalized and expression values modeled using dChip according to default settings (www.dchip.org).
|
| |
|
| Submission date |
Oct 05, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Coleman Lindsley |
| E-mail(s) |
lindsley@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Dr. Kenneth M. Murphy
|
| Street address |
BOX 8118, 660 S. Euclid Ave.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5976 |
Mesp1-induced gene expression changes |
|
| Relations |
| Reanalyzed by |
GSE119085 |
