NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM139029 Query DataSets for GSM139029
Status Public on Jul 01, 2008
Title A2lox.mesp1 (day 6)
Sample type RNA
 
Source name A2lox.mesp1 ES cells
Organism Mus musculus
Characteristics Genotype: Mesp1 transgenic ES cells
Treatment protocol ES cells were differentiated in low density suspension culture.
Growth protocol For differentiation, ES cells were plated in suspension in bacterial petri dishes at 1.5 x 10e4 cells/mL in serum-containing medium (SCM: IMDM with 10% FCS, NEAA, L-glutamine, NaPyruvate, Pen/Strep, and 2-mercaptoethanol) ), and supplemented where indicated with Dkk1 or doxycycline.
Extracted molecule total RNA
Extraction protocol Total RNA was purified using Rneasy mini kits (Qiagen) per manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Description Gene expression data from ES cells differentiated in serum containing medium alone.
Data processing The data were normalized and expression values modeled using dChip according to default settings (www.dchip.org).
 
Submission date Oct 05, 2006
Last update date Aug 28, 2018
Contact name Coleman Lindsley
E-mail(s) lindsley@wustl.edu
Organization name Washington University School of Medicine
Department Pathology and Immunology
Lab Dr. Kenneth M. Murphy
Street address BOX 8118, 660 S. Euclid Ave.
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL1261
Series (1)
GSE5976 Mesp1-induced gene expression changes
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE dChip calculated signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 10.871
AFFX-BioB-M_at 4.69
AFFX-BioB-3_at 8.013
AFFX-BioC-5_at 10.765
AFFX-BioC-3_at 4.736
AFFX-BioDn-5_at 4.204
AFFX-BioDn-3_at 6.203
AFFX-CreX-5_at 5.813
AFFX-CreX-3_at 18.495
AFFX-DapX-5_at 627.297
AFFX-DapX-M_at 1143.14
AFFX-DapX-3_at 1388.503
AFFX-LysX-5_at 66.662
AFFX-LysX-M_at 88.928
AFFX-LysX-3_at 245.026
AFFX-PheX-5_at 122.567
AFFX-PheX-M_at 162.041
AFFX-PheX-3_at 139.469
AFFX-ThrX-5_at 141.378
AFFX-ThrX-M_at 196.411

Total number of rows: 45101

Table truncated, full table size 809 Kbytes.




Supplementary file Size Download File type/resource
GSM139029.CEL.gz 5.8 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap