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Sample GSM1394582 Query DataSets for GSM1394582
Status Public on May 26, 2014
Title thoaric aorta, HPD, biological repl. 2
Sample type RNA
 
Source name thoaric aorta, HPD
Organism Mus musculus
Characteristics tissue: thoracic aorta
strain: DBA/2NCrl
Treatment protocol DBA/2NCrl mice were fed either a high-phosphate diet (HPD) containing 20.2 g phosphorus per kg or a standard chow diet (SCD) containing 7.0 g phosphorus per kg.for 5 days, respectively.
Growth protocol DBA/2NCrl mice were 8 weeks old at the start of the experiment.
Extracted molecule total RNA
Extraction protocol For RNA isolation the aorts were disrupted and homogenized in RLT-buffer (Qiagen)using MagNA lyzer green beads and MagNa lyzer tissue disrupter. Total RNA was isolated using Rneasy Mini Kit (Qiagen) according to the manufacturer´s protocol.
Label biotin
Label protocol NuGen Applause WT Amp Plus ST System and NuGen Biocore Biotin Module
 
Hybridization protocol Affymetrix Mouse Gene 2.0 ST arrays; Affymetrix GeneChip HT Hybridization, Wash and Staining Kit; Affymetrix Fluidics station 450: protocol FS_450_0007 for Cartridge Arrays
Scan protocol Affymetrix scanner: GCS3000
Description Gene expression data from thoracic aortic tissue from mice fed a high phosphate diet
Data processing Affymetrix Geneexpression Console (1.3.1) using Affymetrix default analysis settings for generating CEL.files
Data pre-processing and filtering (Partek Genomic Suite Software v 6.6): RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summerization), ANOVA
 
Submission date May 20, 2014
Last update date May 26, 2014
Contact name Birgit Gallé
Organization name ZMF
Street address Stiftingtalstr. 24
City Graz
ZIP/Postal code 8010
Country Austria
 
Platform ID GPL16570
Series (1)
GSE57818 Impact of high-phosphate diet on aortic gene expression

Data table header descriptions
ID_REF
VALUE RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summerization)

Data table
ID_REF VALUE
17364176 10.8571
17306532 10.3635
17450319 7.16759
17260236 9.67141
17513311 7.81646
17217651 11.342
17380366 11.4903
17548117 9.8065
17225413 7.00982
17458514 7.8897
17298379 11.0592
17233323 10.751
17370807 7.87471
17485789 10.3994
17410845 6.44176
17405075 6.98973
17337585 3.01448
17286962 8.78171
17459716 9.84914
17491368 9.64842

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.




Supplementary file Size Download File type/resource
GSM1394582_TA-HPD-485.CEL.gz 8.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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