|
Status |
Public on Nov 15, 2006 |
Title |
Cells grown in normal media control for folate deficiency, biological rep2 |
Sample type |
RNA |
|
|
Source name |
TK6 grown in normal growth media for 6 days as control for folate deficiency experiment
|
Organism |
Homo sapiens |
Characteristics |
normal human diploid lymphoblast immortalized cell line
|
Extracted molecule |
total RNA |
Extraction protocol |
Ambion miRVANA RNA isolation kit according to manufacturer's protocol
|
Label |
Cy5
|
Label protocol |
The 3’ ends of the RNA molecules were tailed and labeled using the mirVana™ miRNA Labeling Kit (Ambion) according to the manufacturer’s instructions. Amine-modified nucleotides were incorporated during the poly (A) polymerase mediated tailing reaction, and Cy5 succinimide esters (Amersham Biosciences (GE Healthcare)) were conjugated to the amine moieties on the microRNAs
|
|
|
Hybridization protocol |
Labelled miRNA were hybridized for 16 hr at 42C on miRVANA Bioarrays. Following hybridization, bioarrays were washed 3x and dried.
|
Scan protocol |
Bioarrays were scanned using the GenePix 4200AL scanner.
|
Description |
Gene expression data from cells grown in normal media for 6 days as controls for folate deficiency
|
Data processing |
The fluorescent signal associated with the probes and local background was extracted using GenePix Pro and background adjusted fluorescent values were normalized for each microRNA using a variation stabilization transformation method described by Huber et al
|
|
|
Submission date |
Oct 12, 2006 |
Last update date |
Oct 12, 2006 |
Contact name |
Carmen J Marsit |
E-mail(s) |
cmarsit@hsph.harvard.edu
|
Organization name |
Harvard School of Public Health
|
Department |
Genetics and Complex Diseases
|
Lab |
Kelsey
|
Street address |
665 Huntington Avenue
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL4411 |
Series (1) |
GSE6020 |
MiRNA Expression data from Tk6 cell treated with arsenic, gamma-IR, or grown under folate-deficiency |
|