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Sample GSM1416414 Query DataSets for GSM1416414
Status Public on Jul 14, 2014
Title inhouse_low_500pg_rep4
Sample type SRA
Source name inhouse_low_500pg_rep4
Organism Homo sapiens
Characteristics cell line: HEK293T
Extracted molecule total RNA
Extraction protocol cDNA was generated using SMART-seq2 (Picelli et al. Nature Methods 2013)
Tagmentation using in-house Tn5 (low) on 500pg cDNA
cDNA was generated from cells according to Smart-seq2 (Picelli et al. Nature Methods 2013)
We prepared sequencing libraries from cDNA using in-house or commercial Tn5 using tagmentation conditions as outlined in the associated study.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing All reads were aligned to the human (hg19) or mouse (mm10) genomes using STAR (Dobin et al. 2013)
Non-uniquely mapped reads were removed
Rpkmforgenes (available at, options -readcount -fulltranscript -mRNAnorm -rmnameoverlap -u, was used to generate RPKM values and read counts. We used a file with unique positions from Storvall et al. 2013 PLOS ONE. Gene annotations used were transcripts in RefSeq (Feb 2013).
Genome_build: hg19 and mm10
Supplementary_files_format_and_content: RPKM gene expression values (tab-delimited text)
Submission date Jun 19, 2014
Last update date May 15, 2019
Contact name Rickard Sandberg
Organization name Karolinska Institutet
Department Department of Cell and Molecular Biology
Street address Berzelius vag 35
City Stockholm
ZIP/Postal code 17177
Country Sweden
Platform ID GPL11154
Series (1)
GSE58652 Tn5 transposase and tagmentation procedures for massively scaled sequencing projects
BioSample SAMN02867363
SRA SRX611251

Supplementary file Size Download File type/resource
GSM1416414_inhouse_low_500pg_rep4_expression.txt.gz 374.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data included within Sample table

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