|
Status |
Public on Jun 25, 2015 |
Title |
N822 |
Sample type |
RNA |
|
|
Source name |
Tg+ F2 male
|
Organism |
Mus musculus |
Characteristics |
gender: male tissue: mouse prostate tumor tumor_burden: 0.14
|
Treatment protocol |
Prostate tumor sample were collected and snap frozen in liquid nitrogen, then stored at -80 degrees until ready for RNA extraction
|
Growth protocol |
Transgene-positive F2 male tumor growth and metastasis were quantified either at 30 weeks or when humane experimental endpoints were reached.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from tumor samples using QIAGEN Rneasy mini kit
|
Label |
biotin
|
Label protocol |
Labeling performed using GeneChip® Terminal Labeling kit according to manufacturer's protocol.
|
|
|
Hybridization protocol |
The hybridization cocktail containing the fragmented and labeled cDNAs were hybridized to The Affymetrix mouse GeneChip® 2.0 ST chips. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix.
|
Scan protocol |
An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
|
Description |
TRAMPxNOD no metastasis
|
Data processing |
Data was processed using Partek
|
|
|
Submission date |
Jun 25, 2014 |
Last update date |
Oct 09, 2019 |
Contact name |
Kendra Williams |
Organization name |
NIH
|
Department |
NHGRI
|
Lab |
Nigel Crawford
|
Street address |
50 South Dr, Bldg 50
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20814 |
Country |
USA |
|
|
Platform ID |
GPL16570 |
Series (1) |
GSE58829 |
Expression data from prostate tumor of TRAMPxNOD/ShiLtJ F2 cross |
|