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Sample GSM1420271 Query DataSets for GSM1420271
Status Public on Jun 25, 2015
Title N892
Sample type RNA
 
Source name Tg+ F2 male
Organism Mus musculus
Characteristics gender: male
tissue: mouse prostate tumor
tumor_burden: 3.8
Treatment protocol Prostate tumor sample were collected and snap frozen in liquid nitrogen, then stored at -80 degrees until ready for RNA extraction
Growth protocol Transgene-positive F2 male tumor growth and metastasis were quantified either at 30 weeks or when humane experimental endpoints were reached.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from tumor samples using QIAGEN Rneasy mini kit
Label biotin
Label protocol Labeling performed using GeneChip® Terminal Labeling kit according to manufacturer's protocol.
 
Hybridization protocol The hybridization cocktail containing the fragmented and labeled cDNAs were hybridized to The Affymetrix mouse GeneChip® 2.0 ST chips. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix.
Scan protocol An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
Description TRAMPxNOD no metastasis
Data processing Data was processed using Partek
 
Submission date Jun 25, 2014
Last update date Oct 09, 2019
Contact name Kendra Williams
Organization name NIH
Department NHGRI
Lab Nigel Crawford
Street address 50 South Dr, Bldg 50
City Bethesda
State/province MD
ZIP/Postal code 20814
Country USA
 
Platform ID GPL16570
Series (1)
GSE58829 Expression data from prostate tumor of TRAMPxNOD/ShiLtJ F2 cross

Data table header descriptions
ID_REF
VALUE Normalized log2 expression intensity

Data table
ID_REF VALUE
17200001 8.1164
17200003 8.25914
17200005 6.86722
17200007 6.11981
17200009 7.63275
17200011 7.17433
17200013 6.62134
17200015 7.31096
17200017 3.80767
17200019 4.99385
17200021 5.28034
17200023 7.47756
17200025 6.22781
17200027 8.1323
17200029 7.14642
17200031 5.27495
17200033 4.46348
17200035 5.29871
17200037 6.75289
17200039 5.12576

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.




Supplementary file Size Download File type/resource
GSM1420271_N892_MoGene-2_0-st_.CEL.gz 9.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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