|
| Status |
Public on Jun 25, 2015 |
| Title |
N912 |
| Sample type |
RNA |
| |
|
| Source name |
Tg+ F2 male
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
tissue: mouse prostate tumor
tumor_burden: 3.1
|
| Treatment protocol |
Prostate tumor sample were collected and snap frozen in liquid nitrogen, then stored at -80 degrees until ready for RNA extraction
|
| Growth protocol |
Transgene-positive F2 male tumor growth and metastasis were quantified either at 30 weeks or when humane experimental endpoints were reached.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tumor samples using QIAGEN Rneasy mini kit
|
| Label |
biotin
|
| Label protocol |
Labeling performed using GeneChip® Terminal Labeling kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The hybridization cocktail containing the fragmented and labeled cDNAs were hybridized to The Affymetrix mouse GeneChip® 2.0 ST chips. The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix.
|
| Scan protocol |
An Affymetrix Gene Chip Scanner 3000 was used to scan the probe arrays.
|
| Description |
TRAMPxNOD no metastasis
|
| Data processing |
Data was processed using Partek
|
| |
|
| Submission date |
Jun 25, 2014 |
| Last update date |
Oct 09, 2019 |
| Contact name |
Kendra Williams |
| Organization name |
NIH
|
| Department |
NHGRI
|
| Lab |
Nigel Crawford
|
| Street address |
50 South Dr, Bldg 50
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20814 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE58829 |
Expression data from prostate tumor of TRAMPxNOD/ShiLtJ F2 cross |
|
