|
| Status |
Public on Apr 27, 2007 |
| Title |
abnormal eye & brain gene expression patterns in homozygous mbl/axin1 zebrafish - wild-type 48hpf-B |
| Sample type |
RNA |
| |
|
| Source name |
wild-type 48 hr post-fertilization RNA replicate 2
|
| Organism |
Danio rerio |
| Characteristics |
RNA obtained from wild-type zebrafish embryos 48 hours post fertilization
|
| Biomaterial provider |
Han Wang, PhD, University of Oklahoma
|
| Treatment protocol |
Total RNA was obtained from approximately 50 embryos or larvae 48 hours post-fertilization
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Direct labeling incorporating Cy3-dUTP in cDNA from 2 micrograms total RNA with anchored oligo-dT priming and Omniscript reverse transcriptase
|
| |
|
| Hybridization protocol |
The labeled cDNA was added to ChipHybeTM hybridization buffer (Ventana Medical Systems, Tucson) containing Cot-1 DNA (0.5 mg/ml), yeast tRNA (0.2 mg/ml), and poly(dA)40-60 (0.4 mg/ml). Hybridization was performed on a Ventana Discovery system for 9 hr at 58C
|
| Scan protocol |
Agilent DNA Microarray Scanner Model G2505B with fluorescent intensity was measured by Imagene™ software
|
| Description |
none
|
| Data processing |
method of Dozmorov & Centola, Bioinformatics 19:204-211, 2003.
|
| |
|
| Submission date |
Oct 25, 2006 |
| Last update date |
Apr 27, 2007 |
| Contact name |
Bart Frank |
| E-mail(s) |
frank@omrf.org
|
| Organization name |
OMRF
|
| Street address |
825 NE 13 St
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73103 |
| Country |
USA |
| |
|
| Platform ID |
GPL4481 |
| Series (1) |
| GSE6142 |
Gene expression in homozygous mbl/axin1 zebrafish |
|
