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Sample GSM142397 Query DataSets for GSM142397
Status Public on Apr 27, 2007
Title abnormal eye & brain gene expression patterns in homozygous mbl/axin1 zebrafish - wild-type 72hpf-B
Sample type RNA
 
Source name wild-type 72 hr post-fertilization RNA replicate 2
Organism Danio rerio
Characteristics RNA obtained from wild-type zebrafish embryos 72 hours post fertilization
Biomaterial provider Han Wang, PhD, University of Oklahoma
Treatment protocol Total RNA was obtained from approximately 50 embryos or larvae 72 hours post-fertilization
Extracted molecule total RNA
Extraction protocol Trizol
Label Cy3
Label protocol Direct labeling incorporating Cy3-dUTP in cDNA from 2 micrograms total RNA with anchored oligo-dT priming and Omniscript reverse transcriptase
 
Hybridization protocol The labeled cDNA was added to ChipHybeTM hybridization buffer (Ventana Medical Systems, Tucson) containing Cot-1 DNA (0.5 mg/ml), yeast tRNA (0.2 mg/ml), and poly(dA)40-60 (0.4 mg/ml). Hybridization was performed on a Ventana Discovery system for 9 hr at 58C
Scan protocol Agilent DNA Microarray Scanner Model G2505B with fluorescent intensity was measured by Imageneā„¢ software
Description none
Data processing method of Dozmorov & Centola, Bioinformatics 19:204-211, 2003.
 
Submission date Oct 25, 2006
Last update date Apr 27, 2007
Contact name Bart Frank
E-mail(s) frank@omrf.org
Organization name OMRF
Street address 825 NE 13 St
City Oklahoma City
State/province OK
ZIP/Postal code 73103
Country USA
 
Platform ID GPL4481
Series (1)
GSE6142 Gene expression in homozygous mbl/axin1 zebrafish

Data table header descriptions
ID_REF
VALUE normalized/scaled expression level

Data table
ID_REF VALUE
001001001001 1.886328703
001001001002 9.76132E-05
001001001003 9.263807893
001001001004 4.748536384
001001001005 4.806841684
001001001006 3.889658563
001001001007 21.01034642
001001001008 9.76132E-05
001001001009 6.627356812
001001001010 17.20033182
001001001011 0.631895971
001001001012 3.713882202
001001001013 0.077797524
001001001014 48.32119371
001001001015 36.63662099
001001001016 9.081120426
001001001017 8.418690697
001001001018 9.205092215
001001001019 3.564489481
001001001020 2.795538224

Total number of rows: 14720

Table truncated, full table size 358 Kbytes.




Supplementary file Size Download File type/resource
GSM142397.txt.gz 163.8 Kb (ftp)(http) TXT

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